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Monoclonal Anti-AGO1 antibody produced in rat

品牌
Sigma-Aldrich
货号
SAB4200084
规格纯度
~1 mg/mL, clone 4B8, purified immunoglobulin
价格
5045.46 *本价格含增值税费
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半岛bd体育手机客户端 名称:
Monoclonal Anti-AGO1 antibody produced in rat
Anti-Golgi Endoplasmic Reticulum protein 95 kDa
Anti-Eukaryotic Translation Initiation Factor 2c, subunit 1
Anti-Argonaute-1
Anti-GERP95
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti-AGO1 (rat IgG2a isotype) is derived from the hybridoma 4B8 produced by the fusion of mouse myeloma cells (P3X63Ag8.653) and splenocytes from rat immunized with a recombinant human AGO1 fusion protein. The Argonaute family of proteins can be subdivided into the Ago subfamily and the Piwi subfamily. Argonaute proteins have a molecular weight of about 100 kDa and are characterized by piwi-argonaute-zwille (PAZ) and PIWI domains. In human, the Ago subfamily consists of hsAgo1?4 (also known as EIF2C1-4). Ago proteins localize to the cytoplasm of somatic cells and are concentrated in cytoplasmic processing bodies. A member of this group, Ago1 is also known to be associated with Golgi and with endoplasmic reticulum. The gene is located on chromosome 1. Eukaryotic initiation factor (EIF2C1)/hAgo1 is expressed in low to medium levels in most tissues, but its expression is particularly high in embryonic kidney and lung.

免疫原

recombinant human AGO1 fusion protein.

应用

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)

  • Monoclonal Anti-AGO1 antibody produced in rat has been used in:
  • western blotting
  • immunoprecipitation
  • chromatin immunoprecipitation(CHIP) assay
  • RNA immunoprecipitation
  • RNA-nChIP experiments

生化/生理作用

The Argonaute proteins are evolutionarily conserved between species and have been implicated in both transcriptional and post-transcriptional gene silencing. Eukaryotic initiation factor (EIF2C1)/argonaute 1 plays a vital role in activation of transcriptional enhancers and also regulates alternative splicing in human cells. EIF2C1 levels are also increased in tumors that lack the Wilm′s tumor suppressor gene WT1.The encoded protein interacts with hypoxia-responsive microRNAs (HRMs) and stimulate angiogenic pathway.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 200
生物来源 rat
偶联物 unconjugated
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 4B8, monoclonal
形式 buffered aqueous solution
分子量 antigen ~95 kDa
species reactivity mouse, human, canine, bovine, monkey
浓度 ~1 mg/mL
technique(s) immunoprecipitation (IP) : suitable
western blot: 1-2 μg/mL using HEK-293T cell extracts
同位素/亚型 IgG2a
运输 dry ice
储存温度 ?20℃
Gene Information human ... EIF2C1(26523)
mouse ... Eif2c1(236511)

安全信息

储存分类代码 12 - Non Combustible Liquids
闪点(F) Not applicable
闪点(C) Not applicable

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