Anti-LC3 Antibody

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一般描述

LC3A is composed of a heavy chain and multiple light-chain subunits. The molecular machinery required for autophagy is highly conserved in all eukaryotes as seen by the high degree of conservation of proteins involved in the formation of the autophagosome membranes. Recently, both yeast Apg8p and its rat homologue LC3-II were identified as essential constituents of autophagosome membrane as a processed form. In addition, both the yeast and human proteins exist in two modified forms produced by a series of post-translational modifications including a critical C-terminal cleavage after a conserved Gly residue, and the smaller processed form is associated with the autophagosome membranes Results revealed different regulation of the three human isoforms of LC3-II and implicate that the three isoforms may have different physiological functions. Two forms of LC3A, called LC3-I and -II, were produced post-translationally in various cells. LC3-I is cytosolic, whereas LC3-II is membrane bound.

免疫原

Linear peptide corresponding to human LC3.

应用

Research Sub Category
Apoptosis - Additional
Western Blot Analysis: A 1;1,000 dilution from a representative lot detected LC3 in 10 ?g of mouse embryonic fibroblast tissue lysate and MCF-7 cell lysate.
Research Category
Apoptosis & Cancer
Anti-LC3 Antibody is an antibody against LC3 for use in Western Blotting.

质量

Evaluated by Western Blot in NIH/3T3 cell lysate.

Western Blot Analysis: A 1;1,000 dilution of this antibody detected LC3 in 10 ?g of NIH/3T3 cell lysate.

目标描述

~16 kDa observed. LC3 can occur as a doublet around ~15 kDa, indicating the presence of both LC3-I & LC3-II. An uncharacterized band may appear at ~39 and ~55 kDa in some lysates.

联系

Replaces: MABC177; MABC176

外形

Unpurified
Rabbit polyclonal serum containing 0.05% sodium azide.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

分析说明

Control
NIH/3T3 cell lysate

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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