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Anti-DCP2 (C-terminal) antibody produced in rabbit

品牌
Sigma-Aldrich
货号
D6319
规格纯度
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
价格
7319.12 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
Anti-DCP2 (C-terminal) antibody produced in rabbit
Anti-DCP2 decapping enzyme homolog ( S. cerevisiae)
Anti-Nudix motif 20
Anti-NUDT20
Anti-Nucleleoside diphosphate-linked moiety X motif 20
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Dcp2 colocalizes with Dcp1 in distinct cytoplasmic foci along with other proteins involved in the 5′ to 3′ mRNA decay. These foci are termed PB (processing bodies) or DCP-bodies. hDCP2 contains a highly conserved Nudix (nucleoside diphosphate linked to an X moiety) motif critical for the decapping activity.

免疫原

synthetic peptide corresponding to amino acids 406-420 of human DCP2, conjugated to KLH. The corresponding sequence is identical in rat and mouse.

应用

Anti-DCP2 (C-terminal) antibody has been used in immunocytochemistry and image correlation analysis. It may also be used in immunoblotting.
Anti-DCP2 antibody produced in rabbit is suitable for indirect immunofluorescence at a working concentration of 2-5μg/mL using paraformaldehyde-fixed NIH-3T3 cells over-expressing human DCP2 and western blot analysis at a working concentration of 2-4μg/mL using lysates of K-562 and Rat1 cells. Yale Center for High Throughput Cell Biology IF-tested antibodies. Each antibody is tested by immunofluorescence against HUVEC cells using the Yale HTCB IF protocol. To learn more about us and Yale Center for High Throughput Cell Biology partnership, visit sigma.com/htcb-if.

生化/生理作用

Dcp2 is an RNA binding protein and can cleave only a cap structure that is linked to an RNA moiety, suggesting that Dcp2 can differentially associate with specific mRNAs. Dcp2 cleaves the m7G mRNA cap in the 5′ to 3′ mRNA decay pathway, in association with Dcp1 and Hedls complex. Decapping is a critical and highly regulated step in the turnover of mRNA which involves decapping enzymes that hydrolyze the cap structure at the 5′ mRNA. Dcp2 is the catalytic subunit, and the mRNA is degraded by the major cytoplasmic 5′ to 3′ exonuclease XRN1. The enzymatic activity of DCP2 is critically dependent on the DCP1 subunitin vivo.

目标描述

DCP2 (C-terminal) is a key component of an mRNA-decapping complex required for removal of the 5-prime cap from mRNA prior to its degradation from the 5-prime end.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 200
偶联物 unconjugated
抗体形式 affinity isolated antibody
antibody product type primary antibodies
克隆 polyclonal
形式 buffered aqueous solution
分子量 antigen ~50 kDa
species reactivity rat, mouse, human
浓度 ~1 mg/mL
technique(s) indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde-fixed NIH-3T3 celle
indirect immunofluorescence: suitable
western blot: 2-4 μg/mL using lysaes of K-562 and Rat1 cells
UniProt登记号 Q8IU60
运输 dry ice
储存温度 ?20℃
Gene Information human ... DCP2(167227)
mouse ... Dcp2(20640)
rat ... Dcp2(291604)

安全信息

储存分类代码 12 - Non Combustible Liquids
闪点(F) Not applicable
闪点(C) Not applicable
个人防护装备 Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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