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Monoclonal Anti-Microphthalmia antibody produced in mouse

品牌
Sigma-Aldrich
货号
M6065
规格纯度
clone C5, purified immunoglobulin
价格
4741.12 *本价格含增值税费
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半岛bd体育手机客户端 名称:
Monoclonal Anti-Microphthalmia antibody produced in mouse
Anti-Mi
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Microphthalmia (Mi in mouse or MITF in human) is a basic helix-loop-helix-leucine zipper (BHLH–ZIP) transcription factor involved in the differentiation, development and survival of melanocytes and cells of the retinal pigment epithelium, i.e. cells responsible for hair, skin, and eye color. It activates the expression of the melanocyte specific genes tyrosinase and TRP1 (tyrosinase-related protein 1) by binding as a homo- or heterodimer to a symmetrical DNA sequence (E box) (5′-CATGTG-3′) located within the M box found in their promoters. Microphthalmia also appears to be involved in the differentiation of mast cells, osteoclasts, basophils and natural killer cells.
Microphthalmia is expressed in a limited number of cell types including heart, mast cells, osteoclast precursors, and melanocytes. There are a number of different isoforms of microphthalmia resulting from alternative splicing and alternative promotors. These isoforms differ at their amino-termini and in their expression patterns.
Mouse monoclonal clone C5 anti-Microphthalmia antibody recognizes serine phosphorylated and non-phosphorylated melanocytic isoforms of microphthalmia from human, mouse or rat.

免疫原

N-terminal fragment of human microphthalmia protein.

应用

Mouse monoclonal clone C5 anti-Microphthalmia antibody is used to tag serine phosphorylated and non-phosphorylated melanocytic isoforms of microphthalmia for detection and quantitation by techniques such as immunoblotting (doublet of 52-56 kDa), immunoprecipitation, immunohistochemistry on formalin-fixed paraffin embedded or frozen tissue sections, and gel shift. It is used as a probe to determine the roles of microphthalmia in the differentiation, development and survival of melanocytes and cells of the retinal pigment epithelium.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

外形

Solution in phosphate buffered saline containing 0.08% sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号 MFCD00802742
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 200
生物来源 mouse
偶联物 unconjugated
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 C5, monoclonal
形式 buffered aqueous solution
分子量 antigen 52-56 kDa
species reactivity human, mouse, rat
浓度 0.5-1.0 mg/mL
technique(s) immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable using 2 μg/mg protein lysate
western blot: 1:500 using 10 μg of Mouse brain lysates
同位素/亚型 IgG1
UniProt登记号 O75030
运输 wet ice
储存温度 ?20℃
Gene Information human ... MITF(4286)
mouse ... Mitf(17342)
rat ... Mitf(25094)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK nwg
闪点(F) Not applicable
闪点(C) Not applicable

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