半岛bd体育手机客户端 说明

一般描述

Monoclonal Anti-Gravin (mouse IgG2a isotype) is derived from the hybridoma JP74 produced by the fusion of mouse myeloma cells (X63Ag8.653) and splenocytes from BALB/c mice immunized with fractionated membrane cortex proteins from human neurons [cell lines NT2-N and SK-N-BE(2)]. Gravin protein is a myristoylated high molecular weight cytoplasmic protein expressed in endothelial cells, cultured fibroblasts and osteosarcoma cells and highly expressed in the brain.

特异性

Monoclonal Anti-Gravin antibody is specific for human, rat and mouse gravin (approx. 300 kDa).

免疫原

fractionated membrane cortex proteins from human neuron cell lines NT2-N and SK-N-BE(2).

应用

Anti-Gravin antibody has been used in western blotting immunofluorescence.
Monoclonal Anti-Gravin antibody is suitable for use in immunoblotting (using mpkCCD cells ). The product may also be used for immunocytochemistry and immunoprecipitation.

生化/生理作用

Gravin (or AKAP12) is a scaffolding kinase that functions as a tumor suppressor. This kinase interacts with PKC, PKA and other cell cycle signaling proteins to modulate cell division, growth, differentiation and inflammatory responses. The expression of gravin isoforms (α, β, and γ) is regulated by tissue-specific promoters. Gravin also acts as an autoantigen in myasthenia gravis patients .

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号	MFCD08705585
NACRES	NA.44

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	JP74, monoclonal
形式	buffered aqueous solution
species reactivity	rat, human, mouse
technique(s)	immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: 0.02-0.04 μg/mL using rat brain cytosolic S1 fraction
同位素/亚型	IgG2a
UniProt登记号	Q02952
运输	dry ice
储存温度	?20℃
Gene Information	human ... AKAP12(9590) mouse ... Akap12(83397)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)