您好,欢迎来到半岛电竞官方网址 ! [ 登录] [ 免费注册]
半岛电竞官方网址
位置: 首页> 品牌> Sigma-Aldrich> Anti-Destrin/ADF (GV-13) antibody produced in rabbit

Anti-Destrin/ADF (GV-13) antibody produced in rabbit

品牌
Sigma-Aldrich
货号
D8815
规格纯度
affinity isolated antibody, buffered aqueous solution
价格
5688.25 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Anti-Destrin/ADF (GV-13) antibody produced in rabbit
Anti-Actin Depolymerizing Factor
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Destrin/ADF is usually found in regions containing dynamic actin pools such as the leading edge of migrating cells and neuronal growth cones and may also colocalize in cell nuclei. It is also present in ‘Hirano bodies′ in certain brain neurons of dementia patients.

免疫原

synthetic peptide corresponding to amino acids 153-165 of human destrin/ADF.

应用

Anti-Destrin/ADF antibody produced in rabbit is suitable for immunoblotting at a working dilution of 1:1500 using whole extracts of human A-431 epidermoid carcinoma, rat PC-12 pheochromocytoma, and dog MDCK kidney cells and for indirect immunofluorescence at a working dilution of 1:100 using mouse NIH/3T3 fibroblasts. It was used in a study to label chick neurons treated with antimycin to examine the colocalization of actin, cofilin, and ADF in pMAP (phosphorylated microtubule-associated protein) inclusions after ATP depletion. It was used to specifically detect ADF in a study.

生化/生理作用

Destrin/ADF (Actin Depolymerizing Factor) is a small phosphoinositide-sensitive actin-binding protein capable of depolymerizing actin-filamentsin vitro. It binds stoichiometrically to monomeric G-actin and to actin protomers in filaments in a pH-dependent, Ca2+independent manner. It intercalates between longitudinally associated actin monomers within the filament and distorts its helical twist. It is important for many cellular processes involving actin remodeling such as motility at the leading edge of cells, polarized cell growth, endocytosis, phagocytosis, cellular activation, and cytokinesis.

外形

Solution in phosphate buffered saline containing 1% bovine serum albumin and 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号 MFCD03791500
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 200
偶联物 unconjugated
抗体形式 affinity isolated antibody
antibody product type primary antibodies
克隆 polyclonal
形式 buffered aqueous solution
species reactivity human, mouse, canine, rat
浓度 ~0.5-1.0 mg/mL
technique(s) indirect immunofluorescence: 1:100 using mouse NIH/3T3 fibroblasts
western blot: 1:1,500 using whole extracts of human A-431 epidermoid carcinoma cells, rat PC-12 pheochromocytoma cells, and dog MDCK kidney cells
UniProt登记号 P60981
运输 dry ice
储存温度 ?20℃
Gene Information human ... DSTN(11034)
mouse ... Dstn(56431)
rat ... Dstnl1(296197)

Sigma-Aldrich

推荐半岛bd体育手机客户端
Baidu
map