Anti-Collagen Type II (Ab-1) Mouse mAb (II-4C11)

半岛bd体育手机客户端 说明

一般描述

Purified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with P3-X-63-Ag8-LUI mouse myeloma cells. Recognizes the ~130 kDa type II collagen protein.
Recognizes the ~130 kDa type II collagen protein. May react with other collagens. When used at high concentrations higher bands corresponding to aggregates may also be detected.
This Anti-Collagen Type II (Ab-1) Mouse mAb (II-4C11) is validated for use in Frozen Sections for the detection of Collagen Type II (Ab-1).

免疫原

Human
purified collagen type II

包装

100 μg in Plastic Bag(s)
Please refer to vial label for lot-specific concentration.

应用

Frozen Sections (1-10 ?g/ml)

Immunoblotting (1-5 ?g/ml, see application references)

Paraffin Sections (1-10 ?g/ml, see application references)

警告

Toxicity: Standard Handling (A)

外形

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0.

重悬

Following initial thaw, aliquot and freeze (-20°C).

其他说明

May react with other collagens. When used at high concentrations higher bands corresponding to aggregates may also be detected. This antibody may also react with other classes of collagen at a concentration higher than 1 ?g/ml. In immunoblotting, a major band of ~130 kDa is obtained. Antibody should be titrated for optimal results in individual systems.
Kumagai, J. et al. 1994J. Anat.185,278.
Kivrikko, K,I. 1993Ann. Med.25,113.
Sandell, L.J., et al. 1991J. Cell. Biol.114,1307.
Ryan, M.C., and Sandell, L.J. 1990J. Biol. Chem.265,10334.
Mayne, R., and Burgeson, R.E. 1987Structure and Function of Collagen Types,Academic Press, Orlando, FL

法律信息

Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	II-4C11, monoclonal
形式	liquid
包含	≤0.1% sodium azide as preservative
species reactivity	rat, rabbit, human, bovine
manufacturer/tradename	Calbiochem?
储存条件	OK to freeze avoid repeated freeze/thaw cycles
同位素/亚型	IgG1
运输	wet ice
储存温度	?20℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable