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Anti-CEP135

品牌
Sigma-Aldrich
货号
ABE1857
规格纯度
from rabbit
价格
1415.25 *本价格含增值税费
促销
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数量
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半岛bd体育手机客户端 名称:
Anti-CEP135
Centrosomal protoein of 135 kDa, Centrosomal protein 4
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Centrosomal protein of 135 kDa (UniProt: Q66GS9; also known as Cep135, Centrosomal protein 4) is encoded by the CEP135 (also known as CEP4, KIAA0635) gene (Gene ID: 9662) in human. Cep135 is a centrosomal protein that is involved in centriole biogenesis. Human Cep135 is reported to contain two-stranded coiled-coil domains that are critical for microtubule binding. Cep135 acts as a scaffolding protein during early centriole biogenesis and is required for the targeting of centriole satellite proteins to centrosomes such as of PCM1, SSX2IP and CEP290 and recruitment of WRAP73 to centrioles. It also plays a role in centriole-centriole cohesion during interphase by acting as a platform protein for CEP250 at the centriole. Mutations in CEP135 gene are associated with autosomal recessive primary microcephaly-8, a disease characterized by intellectual disability, markedly reduced brain weight, and small cerebral cortex.

特异性

This polyclonal antibody detects centrosomal protein of 135 kDa in human cells. It targets an epitope within 492 amino acids from the C-terminal half.

免疫原

His-tagged recombinant fragment corresponding to 492 amino acids from the C-termnal region of human Cep135.

应用

Anti-CEP135, Cat. No. ABE1857, is a highly specific rabbit polyclonal antibody that targets Centrosomal protein of 135 kDa and has been tested in Electron Microscopy, Immunocytochemistry, and Western Blotting.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected CEP135 in synchronized U2OS cells.

Electron Microscopy Analysis: A representative lot detected CEP135 in Electron Microscopy applications (Kleylein-Sohn, J., et. al. (2007). Dev Cell. 13(2):190-202).

Immunocytochemistry Analysis: A representative lot detected CEP135 in Immunocytochemistry applications (Kleylein-Sohn, J., et. al. (2007). Dev Cell. 13(2):190-202; Guderian, G., et. al. (2010). J Cell Sci. 123(Pt 13):2163-9; Sonnen, K.F., et. al. (2012). Biol Open. 1(10):965-76).

质量

Evaluated by Western Blotting in U2OS cell lysate.

Western Blotting Analysis: 0.5 ?g/mL of this antibody detected CEP135 in 10 ?g of U2OS cell lysate.

目标描述

~150 kDa observed; 133.49 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Protein A purified
Format: Purified

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 purified antibody
antibody product type primary antibodies
克隆 polyclonal
species reactivity human
包装 antibody small pack of 25 μg
technique(s) electron microscopy: suitable
immunocytochemistry: suitable
western blot: suitable
同位素/亚型 IgG
NCBI登记号 NP_079285
UniProt登记号 Q66GS9
运输 ambient
Gene Information human ... CEP135(9662)

Sigma-Aldrich

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