您好,欢迎来到半岛电竞官方网址 ! [ 登录] [ 免费注册]
半岛电竞官方网址
位置: 首页> 品牌> Sigma-Aldrich> Anti-β-Glucuronidase (C-Terminal) antibody produced in rabbit

Anti-β-Glucuronidase (C-Terminal) antibody produced in rabbit

品牌
Sigma-Aldrich
货号
G5545
规格纯度
~1.5 mg/mL, affinity isolated antibody, buffered aqueous solution
价格
5827.44 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Anti-β-Glucuronidase (C-Terminal) antibody produced in rabbit
Anti-GUS
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

β-Glucuronidase (GUS) gene (also referred to as uidA) fromEscherichia- coli, codes for a 60kDa protein.

特异性

Anit-β-Glucuronidase (C-Terminal) recognizes bacterial GUS expressed in transgenic tobacco plants.
The antibody recognizes bacterial GUS expressed in transgenic tobacco plants.

免疫原

synthetic peptide corresponding to amino acids 589-603 at the C-terminus ofE. coliGUS, conjugated to KLH.

应用

Detection of GUS by immunoblotting (60 kDa). Staining of the GUS band in immunoblotting is specifically inhibited by the immunizing GUS peptide (E. coli, amino acids 589-603).

生化/生理作用

β-Glucuronidase (GUS) acts as a reporter gene for plant studies. Reporter genes are widely used for studying the expression of foreign genes in transformed plant tissues. GUS is an hydrolase that catalyzes the cleavage of a variety of β-glucuronide derivatives available for colorimetric, fluorometric and histochemical assays. GUS activity is easily assayed in vitro and can withstand fixation, enabling histochemical localization in cells and tissue sections. However, one of the major limitations of the gus reporter gene system is that the histochemical GUS assay system is destructive for the plant tissue, and therefore it is not suitable for direct visual selection of transformed plants.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

制备说明

The antibody is affinity-purified using the immunizing peptide immobilized on agarose.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号 MFCD00282142
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 200
偶联物 unconjugated
抗体形式 affinity isolated antibody
antibody product type primary antibodies
克隆 polyclonal
形式 buffered aqueous solution
分子量 antigen 60 kDa
species reactivity plant
浓度 ~1.5 mg/mL
technique(s) western blot: 1-2 μg/mL using purified GUS fromE. coli
运输 dry ice
储存温度 ?20℃

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable

Sigma-Aldrich

推荐半岛bd体育手机客户端
Baidu
map