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Anti-PCAF antibody produced in rabbit

品牌
Sigma-Aldrich
货号
P7493
规格纯度
affinity isolated antibody, buffered aqueous solution
价格
4731.57 *本价格含增值税费
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半岛bd体育手机客户端 名称:
Anti-PCAF antibody produced in rabbit
Anti-p300/CBP associated factor
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

PCAF (p300/CBP associated factor, P/CAF), a type A histone acetyltransferases (HAT), has been identified in several species. Human PCAF is a ubiquitous protein member of the GCN5-related N-acetyltransferase (GNAT) superfamily.

特异性

Anti-PCAF recognizes human and dog PCAF. In some extract preparations, additional band(s) may appear. Staining of PCAF band(s) by immunoblotting is inhibited by the PCAF immunizing peptide.

免疫原

synthetic peptide corresponding to the C-terminal (amino acids 817-832) of human PCAF with N-terminal added cysteine, conjugated to KLH.
This sequence is identical to mouse PCAF and varies by 2 amino acids residues in chicken.

应用

Anti-PCAF antibody is also suitable for use microarray, immunoblot, chemiluminescent western blot (1:500 using whole extracts of dog MDCK kidney cells), and immunoprecipitation (5-10 μg using RIPA lysate with approx. 4x10,000,000 human acute T cell leukemia Jurkat cells).
Anti-PCAF antibody produced in rabbit has been used in western blot and immunoprecipitation.

生化/生理作用

PACF (p300/CBP association factor) functions as a transcriptional coactivator in association with p300 and CBP (CREB-binding protein). PCAF share high degree of sequence similarity with human and mouse GCN5 HAT (Histone acetyltransferases). PACF mediates the acetylation of core histone. It is also known for the acetylation of non-histone proteins such as p53, MyoD, TFIIE-b, TFIIE, GATA-1, EKLF, HMGI (Y), and several other cellular and viral proteins. PCAF possesses chromatin modification activity and modulation of transcription factors for the activation of transcription. It also promotes cell growth and development as myogenesis and also contribute to receptor-mediated cell signaling or activation.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

储存及稳定性

For continuous use, store at 2-8 ℃ for up to one month. For prolonged storage, freeze in working aliquots at -20 ℃. Repeated freezing and thawing, or storage in frost-free freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号 MFCD03791929
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 200
偶联物 unconjugated
抗体形式 affinity isolated antibody
antibody product type primary antibodies
克隆 polyclonal
形式 buffered aqueous solution
分子量 antigen ~88 kDa
species reactivity human, canine
technique(s) immunoprecipitation (IP): 5-10 μg using RIPA lysate with ~4x107human acute T cell leukemia Jurkat cells
microarray: suitable
western blot (chemiluminescent): 1:500 using whole extracts of dog MDCK kidney cells
UniProt登记号 Q92831
运输 dry ice
储存温度 ?20℃
Gene Information human ... KAT2B(8850)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK nwg
闪点(F) Not applicable
闪点(C) Not applicable

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