Anti-NKX6.2

半岛bd体育手机客户端 说明

一般描述

Homeobox protein Nkx-6.2 (UniProt Q9C056; also known as Homeobox protein NK-6 homolog B) is encoded by the NKX6-2 (also known as GTX, NKX6B) gene (Gene ID 84504) in human. Mammalian NKX gene products and their orthologs in other species are known to regulators of neuronal cell fate. Both the ventral nervous system defective (vnd) gene product in Drosophila and murine NKX proteins in spinal cord direct progenitors to adopt specific ventral neural identities. In mice, NKX6.2 is expressed in a small subset of ventral neural progenitors in a restricted spatial pattern at the border between the medial and caudal ganglionic eminence (MGE and CGE). NKX6,2 is required for specification of V1 interneurons in the spinal cord and progenitors are found to adopt a more dorsal V0 identity in the absence of NKX6,2. In addition, NKX6.2-positive brain region is shown to give rise to both parvalbumin (PV) and somatostatin (SST) cortical interneurons and in particular be enriched in a subpopulation of SST cortical interneurons that coexpress calretinin (CR).

特异性

This rabbit polyclonal antibody detected the exogenously expressed human NKX6.2 in transfected HEK293T cells, while no immunoreactive bands were detected in lysates from untransfected cells. The immunogen sequence lies within the region C-terminal to the Homeobox and is 100% conserved among human, mouse, and rat species.

免疫原

KLH-conjugated linear peptide corresponding to a C-terminal region sequence of human/mouse/rat NKX6.2.

应用

Anti-NKX6.2, Cat. No. ABN1455, is a highly specific rabbit polyclonal antibody that targets Homeobox protein Nkx-6.2 and has been tested in Immunohistochemistry (Paraffin) and Western Blotting.
Research Category
Neuroscience
Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected NKX6.2 in human cerebral cortex and kidney tissue sections.

Western Blotting Analysis: 1 ?g/mL from a representative lot detected Myc-DDK-tagged human NKX6.2 in 10 ?g of lysate from transfected HEK293T cells.

质量

Evaluated by Immunohistochemistry in human pancreas tissue.

Immunohistochemistry Analysis: A 1:1,000 dilution of this antibody detected NKX6.2 in human pancreas tissue section.

目标描述

~29 kDa observed. 29.26 kDa (human) and 29.36 kDa (mouse and rat) calculated. Uncharacterized bands may be observed in some lysate(s).

外形

Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss

32160702

半岛bd体育手机客户端 性质

生物来源	rabbit
质量水平	100
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
纯化方式	affinity chromatography
species reactivity	human
species reactivity (predicted by homology)	rat (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), mouse (based on 100% sequence homology)
technique(s)	immunohistochemistry: suitable (paraffin) western blot: suitable
NCBI登记号	NP_796374
UniProt登记号	Q9C056
运输	ambient

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable