Anti-Dicer antibody produced in rabbit

半岛bd体育手机客户端 说明

一般描述

Dicer, also known as Dicer1, endoribonuclease Dicer, Helicase with RNase motif is a member of the RNase III family. It contains a N-terminal DEXH-box RNA helicase/ ATPase domain, followed by a domain of unknown function (DUF283), a Piwi/Argonaut/Zwill (PAZ ) domain, which anchors the 3′-end of RNA substrates.
Dicer1 is encoded by the gene mapped to human chromosome 14q32.13.

特异性

Anti-Dicer recognizes human Dicer.

应用

Anti-Dicer antibody produced in rabbit has been used in:

• immunoblotting
• immunoprecipitation
• immunofluorescent staining

生化/生理作用

Dicer catalyzes the first step in the RNA interference (RNAi) pathway and initiates formation of the RNA-induced silencing complex (RISC). Dicer processes the dsRNA into small fragments called short interfering RNA (siRNA) or microRNA (miRNA) of typically 21-25 nucleotides long with a two-base overhang on the 3′. It helps to load these fragments into the RNA-induced silencing complex (RISC), which guides the sequence-specific silencing of mRNAs that contain complementary sequences by either enzymatically cleaving the mRNA or repressing its translation.
Mutation in the Dicer gene causes malignancies.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

储存及稳定性

Store at –20 ℃. For continuous use, store at 2–8 ℃ for up to one month. For extended storage, freeze in working aliquots at –20 ℃. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES

NA.41

半岛bd体育手机客户端 性质

生物来源	rabbit
偶联物	unconjugated
抗体形式	affinity isolated antibody
antibody product type	primary antibodies
克隆	polyclonal
形式	buffered aqueous solution
分子量	antigen ~218 kDa
浓度	~1.0 mg/mL
technique(s)	immunoprecipitation (IP): 2.5-5 μg using HeLa cell lysates indirect immunofluorescence: 2-5 μg/mL using paraformaldehyde fixed HeLa cells western blot: 3-6 μg/mL using HeLa cell lysates
运输	dry ice
储存温度	?20℃
Gene Information	human ... DICER1(23405) mouse ... Dicer1(192119)

安全信息

储存分类代码	12 - Non Combustible Liquids
闪点(F)	Not applicable
闪点(C)	Not applicable