您好,欢迎来到半岛电竞官方网址 ! [ 登录] [ 免费注册]
半岛电竞官方网址
位置: 首页> 品牌> Sigma-Aldrich> Anti-PABP antibody, Mouse monoclonal antibody produced in mouse

Anti-PABP antibody, Mouse monoclonal antibody produced in mouse

品牌
Sigma-Aldrich
货号
P6246
规格纯度
clone 10E10, purified from hybridoma cell culture
价格
4731.57 *本价格含增值税费
促销
服务
  • 原厂保证
  • 包邮
  • 增值税票
数量
- +
半岛bd体育手机客户端 名称:
Anti-PABP antibody, Mouse monoclonal antibody produced in mouse
Anti-Poly(A)-Binding Protein
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Poly(A) binding protein (PABP) possesses RNA recognition motifs (RRMs), a proline-rich region and a PABP C-terminal domain. The gene encoding this protein is localized on human chromosome 8q22.3.
Monoclonal Anti-PABP (mouse IgG1 isotype) is derived from the 10E10 hybridoma produced by the fusion of mouse myeloma cells (SP2/0) and splenocytes from BALB/c mice immunized with recombinant human PABP. Poly(A) binding protein (PABP) is highly conserved between different organisms especially at the amino-terminal region of the protein that contains four RNA-binding domains (RBDs).

免疫原

recombinant human PABP.

应用

Monoclonal Anti-PABP antibody produced in mouse has been used for immunofluorescence.
Monoclonal Anti-PABP antibody produced in mouse has been used in:

  • Immunoblotting
  • Immunostaining
  • Immunoprecipitation
  • Enzyme linked immunosorbent assay(ELISA)
  • Immunocytochemistry

生化/生理作用

Poly(A) binding protein (PABP) is found in excess, relative to the amount of cytoplasmic poly(A) (three fold) and binds to its poly(A) target at high affinity (Kd of 7nM). The eukaryotic initiation factor, EIF4F complex, interacts with PABP indirectly via the PAIP-1 protein (PABP-interacting protein-1). As a consequence, an interaction between the 5′ and 3′ ends of mRNA occurs. This proximity between the mRNA ends contributes to its stability and enhances translation, since terminating ribosomes may start translation again.
Poly(A) binding protein (PABP) has a role in mRNA translation and stability. It modulates gene expression. The protein binds to the poly(A) tail, bridges the ends of mRNA and forms a closed loop. This might help in translation initiation by promoting ribosome recruitment.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号 MFCD06412253
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 200
生物来源 mouse
偶联物 unconjugated
抗体形式 purified from hybridoma cell culture
antibody product type primary antibodies
克隆 10E10, monoclonal
形式 buffered aqueous solution
分子量 antigen ~69 kDa
species reactivity canine, monkey,Xenopus, bovine, hamster, human
technique(s) immunocytochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.5-1 μg/mL using whole cell extracts of HEK 293T cells
同位素/亚型 IgG1
UniProt登记号 P11940
运输 dry ice
储存温度 ?20℃
Gene Information human ... PABPC1(26986)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK nwg
闪点(F) Not applicable
闪点(C) Not applicable

Sigma-Aldrich

推荐半岛bd体育手机客户端
Baidu
map