Anti-Neuron-Specific Enolase (NSE), Mouse monoclonal

半岛bd体育手机客户端 说明

一般描述

Monoclonal anti-neuron-specific enolase (NSE) (mouse IgG1 isotype) is derived from the hybridoma NSE-P1 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Neuron specific enolase (NSE) belongs to the family of enolase enzymes. Enolases have three subunits (a, b, and g), which can combine to form five different isoenzymes: aa, ab, ag, bb, and gg. Enolase 1 (aa) is present in adipose tissue, kidney, liver, and spleen. Enolase 3 (bb) is muscle specific. Enolase 2 (ENO2), also known as neuron-specific enolase (NSE) is a cytoplasmic enzyme, that is released during cell destruction and has a half-life of approximately 30 hours in serum. This dimeric enzyme (gg) is found in neurons and in neuroendocrine cells. ENO2 gene is mapped to human chromosome 12p13.

特异性

Monoclonal Anti- Neuron-Specific Enolase (NSE) recognizes human, rat, and mouse NSE.

免疫原

synthetic peptide corresponding to a sequence at the C-terminal region of human NSE.¹The isotype is determined by ELISA using Mouse Monoclonal Antibody Isotyping Reagents (Sigma ISO-2).

应用

Anti-Neuron-Specific Enolase (NSE), Mouse monoclonal has been used in:

• immunofluorescence staining
• immunocytochemistry
• immunofluorescence microscopy
• immunoblotting
• enzyme-linked immunosorbent assay (ELISA)
• immunohistochemistry

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunocytochemistry (1 paper)

生化/生理作用

Enolase 2 (ENO2) participates in glycolysis by converting β-glycerophosphate into dihydroxyacetone phosphate. It serves as a neurobiochemical marker of brain damage after traumatic brain injury, anoxic encephalopathy, stroke, and cardiac arrest. NSE also acts as a circulating marker for neuroendocrine tumors. High levels of NSE is observed in breast cancer upon exposure to arsenite and cadmium.

外形

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

储存及稳定性

For extended storage, freeze at –20 ℃ in working aliquots. Repeated freezing and thawing or storage in “frost-free” freezers is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

NACRES

NA.41

半岛bd体育手机客户端 性质

质量水平	200
生物来源	mouse
偶联物	unconjugated
抗体形式	purified from hybridoma cell culture
antibody product type	primary antibodies
克隆	NSE-P1, monoclonal
形式	buffered aqueous solution
分子量	antigen ~47 kDa
species reactivity	human, rat, mouse
浓度	~1.0 mg/mL
technique(s)	immunohistochemistry: 10-20 μg/mL using formalin-fixed paraffin embedded human cerebellum. western blot: 0.5-1.0 μg/mL using NTERA-2 (NT2/D1) total cell extracts.
同位素/亚型	IgG1
UniProt登记号	P09104
运输	dry ice
储存温度	?20℃
Gene Information	human ... ENO2(2026)

安全信息

储存分类代码	12 - Non Combustible Liquids
闪点(F)	Not applicable
闪点(C)	Not applicable