半岛bd体育手机客户端 说明
一般描述
MANF (mesencephalic astrocyte-derived neurotrophic factor) forms a family of neurotrophic factors (NTFs) along with cerebral dopamine neurotrophic factor (CDNF). It is characterized by eight conserved cysteine residues, a saposin-like domain in the N-terminal and a putative unfolded carboxy-terminal domain. This protein has a 21 amino acids long signal peptide, which is cleaved off to yield a mature protein of 158 amino acids. MANF is a secreted protein, but some of it remains in endoplasmic reticulum (ER). It was initially isolated from cultured embryonic dopaminergic neurons, and in brain, is predominantly expressed in cerebral cortex, hippocampus and cerebellar Purkinje cells. This gene is localized to human chromosome 3, spans 4.3kb, and contains 4 exons. The encoded protein has a molecular weight of 20kDa.
免疫原
Protein ARMET precursor recombinant protein epitope signature tag (PrEST)
应用
All Prestige Antibodies Powered by Atlas Antibodies are developed and validated by the Human Protein Atlas (HPA) project (www.proteinatlas.org)and as a result, are supported by the most extensive characterization in the industry.
The Human Protein Atlas project can be subdivided into three efforts: Human Tissue Atlas, Cancer Atlas, and Human Cell Atlas. The antibodies that have been generated in support of the Tissue and Cancer Atlas projects have been tested by immunohistochemistry against hundreds of normal and disease tissues and through the recent efforts of the Human Cell Atlas project, many have been characterized by immunofluorescence to map the human proteome not only at the tissue level but now at the subcellular level. These images and the collection of this vast data set can be viewed on the Human Protein Atlas (HPA) site by clicking on the Image Gallery link. To view these protocols and other useful information about Prestige Antibodies and the HPA, visit sigma.com/prestige.
生化/生理作用
MANF (mesencephalic astrocyte-derived neurotrophic factor) plays a protective role towards midbrain dopaminergic neurons. It is an endoplasmic reticulum (ER) stress related protein. Its levels are increased post brain injury, ischemia and epileptic insults. It might confer neuro-protection against brain ischemia, by preventing necrosis or apoptosis of cerebral cortex cells. It is over-expressed during unfolded protein response (UPR), and prevents ER stress-induced apoptosis, as well as cell proliferation.
特点和优势
Prestige Antibodies?are highly characterized and extensively validated antibodies with the added benefit of all available characterization data for each target being accessible via the Human Protein Atlas portal linked just below the product name at the top of this page. The uniqueness and low cross-reactivity of the Prestige Antibodies?to other proteins are due to a thorough selection of antigen regions, affinity purification, and stringent selection. Prestige antigen controls are available for every corresponding Prestige Antibody and can be found in the linkage section.
Every Prestige Antibody is tested in the following ways:
联系
Corresponding Antigen APREST72053.
外形
Solution in phosphate-buffered saline, pH 7.2, containing 40% glycerol and 0.02% sodium azide
法律信息
Prestige Antibodies is a registered trademark of sigma-aldrich Co. LLC
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
| 人类蛋白质图谱编号 | HPA011175 Human Protein Atlas characterization data |
半岛bd体育手机客户端 性质
| 生物来源 | rabbit |
| 质量水平 | 100 |
| 偶联物 | unconjugated |
| 抗体形式 | affinity isolated antibody |
| antibody product type | primary antibodies |
| 克隆 | polyclonal |
| 半岛bd体育手机客户端 线 | Prestige Antibodies?Powered by Atlas Antibodies |
| 形式 | buffered aqueous glycerol solution |
| species reactivity | human |
| 包装 | antibody small pack of 25 μL |
| 增强验证 | orthogonal RNAseq Learn more about Antibody Enhanced Validation |
| technique(s) | immunoblotting: 0.04-0.4 μg/mL immunofluorescence: 0.25-2 μg/mL immunohistochemistry: 1:200-1:500 |
| 免疫原序列 | CEVCISYLGRFYQDLKDRDVTFSPATIENELIKFCREAR |
| 运输 | wet ice |
| 储存温度 | ?20℃ |
| Gene Information | human ... ARMET(7873) |
安全信息
| 储存分类代码 | 12 - Non Combustible Liquids |
| WGK | WGK 1 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
| 个人防护装备 | Eyeshields, Gloves, multi-purpose combination respirator cartridge (US) |
Sigma-Aldrich
m.cnreagent.com
