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Anti-LRRK2 Antibody

品牌
Sigma-Aldrich
货号
ABN187
规格纯度
from rabbit, purified by affinity chromatography
价格
6022.61 *本价格含增值税费
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半岛bd体育手机客户端 名称:
Anti-LRRK2 Antibody
Leucine-rich repeat serine/threonine-protein kinase 2, Dardarin, JH5514
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

LRRK2 (Leucine-rich repeat serine/threonine-protein kinase 2) is a multifunctional protein belonging to the ROCO family of proteins. LRRK2 contains multiple domains including a ROC (Ras of Complex protein) domain that binds and hydrolyzes GTP, and a kinase domain involved in the phosphorylation of myelin basic protein (MBP), mosein, ezrin, and radixin. LRRK2 itself undergoes autophosphorylation and is targeted by other kinases that modify multiple serine residues at its N-terminus; It is constitutively phosphorylated at Ser935 and maintains this state by interaction with the 14-3-3 protein. Although the kinases upstream of LRRK2 have not been definitively identified, previous studies have suggested that this group of kinases may include PKA. It also contains multiple leucine-rich repeat motifs that facilitate interaction with a range of proteins including PARK2 and PRDX3. Mutations in LRRK2, most of which have enhanced its kinase activity, may contribute to the development of late-onset familial Parkinson’s disease; however, the precise molecular mechanism of LRRK2-induced Parkinson’s disease is still unclear.

免疫原

KLH-conjugated linear peptide corresponding to human LRRK2.

应用

This Anti-LRRK2 Antibody is validated for use in WB, IH(P) for the detection of LRRK2. This antibody is known as JH5514 in scientific publications.
Research Category
Neuroscience
Research Sub Category
Neurodegenerative Diseases
Western Blot Analysis: A representative lot was used by an independent laboratory in HEK-293T cells transiently transfected with myc-tagged WT or mutant LRRK2. (West, A., et al. (2005). PNAS. 102(46):16842–16847.)

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in neurons from mouse forebrain tissue. (Higashi, S., et al. (2007). Journal of Neurochemistry. 100:368–381.)

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in transfected HEK-293T cells. (West, A., et al. (2005). PNAS. 102(46):16842–16847.)

Immunofluorescence Analysis: A representative lot was used by an independent laboratory in transfected HEK-293T cells. (West, A., et al. (2005). PNAS. 102(46):16842–16847.)

质量

Evaluated by Western Blot in mouse brain tissue lysate.

Western Blot Analysis: 1 ?g/mL of this antibody detected LRRK2 in 10 ?g of mouse brain tissue lysate.

目标描述

~290 kDa observed

外形

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Affinity purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
Mouse brain tissue lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 affinity isolated antibody
antibody product type primary antibodies
克隆 polyclonal
纯化方式 affinity chromatography
species reactivity human, mouse
technique(s) immunofluorescence: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
NCBI登记号 NP_940980
UniProt登记号 Q5S007
运输 wet ice
Gene Information human ... LRRK2(120892)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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