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Anti-GADD45 alpha Antibody

品牌
Sigma-Aldrich
货号
ABE2696
规格纯度
from rabbit, purified by affinity chromatography
价格
5688.25 *本价格含增值税费
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数量
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半岛bd体育手机客户端 名称:
Anti-GADD45 alpha Antibody
Growth arrest and DNA damage-inducible protein GADD45 alpha, DDIT-1, DNA damage-inducible transcript 1 protein
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Growth arrest and DNA damage-inducible protein GADD45 alpha (UniProt P24522; also known as DDIT-1, DNA damage-inducible transcript 1 protein) is encoded by the GADD45A (also known as DDIT1, GADD45) gene (Gene ID 1647) in human. GADD45A, GADD45B, and GADD45G constitute a family of sensors that can mediate rapid responses to a variety of stressors in mammals. GADD45 proteins are involved in cellular DNA repair, cell cycle, cell survival, apoptosis, senescence, and DNA demethylation under various stress conditions. The interaction of GADD45 with PCNA regulates DNA replication and repair by stimulating nucleotide excision repair. GADD45 can induce cell cycle arrest through direct interaction with cell cycle-related protein CDK1 and p21. Direct phosphorylation of MEKK4 by GADD45 leads to activation of JNK and p38 kinase, resulting in cellular apoptosis and senescence, respectively. All GADD45 proteins are able to interact with each other and form homo- or hetero-dimers, which are crucial for GADD45 functions. GADD45 proteins are ubiquitously expressed, but can vary in their tissue expression patterns among the three members. Gadd45a deletion in mice causes genomic instability, resulting in defective mitosis and cytokinesis, as wll as increased radiation carcinogenesis. In addition to the reported antitumor activity via the induction of cellular apoptosis or senescence in cancer cells, GADD45A has also been shown to play a role in facilitating tumorigenesis in certain cancers.

特异性

This polyclonal antibody detects GADD45 alpha. Immunogen sequence is not conserved in GADD45 beta.

免疫原

KLH-conjugated linear peptide corresponding to the N-terminal sequence of human GADD45 alpha.
Epitope: N-terminus.

应用

This Anti-GADD45 alpha Antibody is validated for use in Western Blotting, Immunohistochemistry for the detection of GADD45 alpha.
Research Sub Category
Cell Cycle, DNA Replication & Repair
Western Blotting Analysis: A 1:500 dilution from a representative lot detected GADD45 alpha dimer in 10 ?g of HEK293 cell lysate.
Immunohistochemistry Analysis: A 1:250 dilution from a representative lot detected GADD45 alpha in human colon cancer tissue section.
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blotting in mouse kidney tissue lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected GADD45 alpha in 10 ?g of mouse kidney tissue lysate.

目标描述

~18/36 kDa (monomer/dimer) observed. 18.34 kDa (monomer) calculated. Uncharacterized band(s) may appear in some lysates.

联系

Replaces: 07-1230

外形

Affinity purified.
Purified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

其他说明

Concentration: Please refer to lot specific datasheet.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 100
抗体形式 affinity isolated antibody
antibody product type primary antibodies
克隆 polyclonal
纯化方式 affinity chromatography
species reactivity mouse, human
species reactivity (predicted by homology) bovine (based on 100% sequence homology), porcine (based on 100% sequence homology)
technique(s) immunohistochemistry: suitable
western blot: suitable
NCBI登记号 NP_001915
UniProt登记号 P24522
Gene Information human ... GADD45A(1647)

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 1
闪点(F) Not applicable
闪点(C) Not applicable

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