半岛bd体育手机客户端 说明
一般描述
TMPK, also known as dTMP kinase, and encoded by the gene DTYMK/CDC8/TMPK/TYMK, is a key enzyme for pyrimidine synthesis that catalyzes the phosphorylation of thymidine 5′-monophosphate (dTMP) to form thymidine 5′-diphosphate (dTDP). TMPK has important functions in cell proliferation and DNA replication, and the synthesis of dTDP is unique because it requires TMPK. All other dNDPs are directly produced by ribonucleotide reductase (RNR). Thus, TMPK is well recognized as a potential drug target for many diseases and disease agents including viruses. TMPK is currently a target of anti-HIV nucleoside therapy, and TMPK is also a validated target for antibiotic development against resistance strains of bacteria and parasites, and research demonstrates that it may also be a good target for the anticancer agent doxorubicin cancer cells. Expressed by virtually all cells it is localized to cell cytoplasm with only traces found in Golgi, mitochondria and nuclei.
免疫原
GST-tagged recombinant protein corresponding to human Thymidylate Kinase/TMPK.
应用
Research Sub Category
Apoptosis - Additional
Anti-Thymidylate Kinase/TMPK Antibody is a rabbit polyclonal antibody, validated for use in western blotting & IP.
Western Blotting Analysis: A representative lot from an independent laboratory detected Thymidylate Kinase/TMPK in MDA-MB231, MCF-7, H184B5F5/M10 cell lysates (Hu, C. M., et al. (2012). Cancer Cell. 22(1):36-50.).
Immunoprecipitation Analysis: A representative lot from an independent laboratory immunprecipitated Thymidylate Kinase/TMPK from MCF-7, H184B4F5/M10, and MCF-10A cell lysates (Ke, P. Y., et al. (2005). Genes Dev. 19(16):1920-1933.).
Western Blotting Analysis: A representative lot from an independent laboratory detected Thymidylate Kinase/TMPK in MCF-7, H184B4F5/M10, and MCF-10A cell lysates (Ke, P. Y., et al. (2005). Genes Dev. 19(16):1920-1933.).
Research Category
Apoptosis & Cancer
质量
Evaluated by Western Blotting in Jurkat cell lysate.
Western Blotting Analysis: 2 ?g/mL of this antibody detected Thymidylate Kinase/TMPK in 10 ?g of Jurkat cell lysate.
目标描述
~24 kDa observed
外形
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Format: Purified
Protein A purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | human |
technique(s) | immunoprecipitation (IP): suitable western blot: suitable |
NCBI登记号 | NP_036277 |
UniProt登记号 | P23919 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich