Anti-PKM2 Antibody

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一般描述

Pyruvate kinase isozymes M1/M2 (PKM2) is also called Cytosolic thyroid hormone-binding protein (CTHBP), Opa-interacting protein 3 (OIP-3), Pyruvate kinase 2/3, Pyruvate kinase muscle isozyme, Thyroid hormone-binding protein 1 (THBP1) and p58. PKM2 is a glycolytic enzyme that assists in generating ATP. PKM2 also stimulates POU5F1-mediated transcriptional activation, and plays a role in caspase independent cell death of tumor cells. The ratio between the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. This control of glycolysis and is important for tumor cell proliferation and survival. PKM2 is specifically expressed in proliferating cells, such as embryonic stem cells, embryonic carcinoma cells, and cancer cells.

特异性

This antibody is specific to PKM2.

免疫原

Linear peptide corresponding to human PKM2.

应用

Research Sub Category
Apoptosis - Additional
Research Category
Apoptosis & Cancer
Anti-PKM2 Antibody detects level of PKM2 & has been published & validated for use in WB and IHC.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected PKM2 in 10 ?g of C2C12, HEK293, HeLa, HepG2, human placenta, HUVEC, Jurkat, L6, mouse brain, NIH/3T3, PC12, PC3, and RAW264.7 lysate.
Western Blotting Analysis: A representative lot detected PKM2 in HEK293 cell lysate (Christofk, R. H., et al. (2008). Nature. 452:230-233).
Immunohistochemistry Analysis: A representative lot detected PKM2 in HEK293 tissue (Christofk, R. H., et al. (2008). Nature. 452:230-233).

质量

Evaluated by Western Blotting in A431 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected PKM2 in 10 ?g of A431 cell lysate.

目标描述

~ 58 kDa observed

外形

Rabbit polyclonal serum with 0.05% sodium azide.
Unpurified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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