半岛bd体育手机客户端 说明
一般描述
The spatial coordination of neurotransmitter receptors with other postsynaptic signaling and structural molecules is regulated by a diverse array of cell-specific scaffolding proteins. The synaptic trafficking of AMPA receptors by the stargazin protein in some neurons, for example, depends on specific interactions between the C terminus of stargazin and the PDZ [postsynaptic density-95 (PSD-95)/Discs large/zona occludens-1] domains of membrane-associated guanylate kinase scaffolding proteins PSD-93 or PSD-95. Stargazin [Cacng2 (Ca2+ channel gamma2 subunit)] is one of four closely related proteins recently categorized as transmembrane AMPA receptor regulating proteins (TARPs) that appear to share similar functions but exhibit distinct expression patterns in the CNS. MAGI-2 (membrane associated guanylate kinase, WW and PDZ domain containing 2) has been identified as a novel candidate interactor with the cytoplasmic C termini of the TARPs. MAGI-2 [also known as S-SCAM (synaptic scaffolding molecule)] is a multi-PDZ domain scaffolding protein that interacts with several different ligands in brain, including PTEN (phosphatase and tensin homolog), dasm1 (dendrite arborization and synapse maturation 1), dendrin, axin, beta- and delta-catenin, neuroligin, hyperpolarization-activated cation channels, beta1-adrenergic receptors, and NMDA receptors. MAGI-2 coimmunoprecipitates with stargazin in vivo. Expression of stargazin recruites MAGI-2 to cell membranes and cell-cell contact sites in transfected HEK-293T cells dependent on the presence of the stargazin -TTPV motif. MAGI-2 represents a strong candidate for linking TARP/AMPA receptor complexes to a wide range of other postsynaptic molecules and pathways and advance our knowledge of protein interactions at mammalian CNS synapses.
特异性
Reactivity with other species has not been determined.
Cat. # AB9878 recognizes MAGI-2
免疫原
GST-tagged MAGI-2
应用
This Anti-MAGI-2 Antibody is validated for use in IC, IH, IP, WB for the detection of MAGI-2.
质量
Routinely evaluated by Western Blotting on mouse brain tissue lysate.
Western Blotting Analysis: 1:500 dilution of this lot was used to detect MAGI-2 on mouse brain lysate.
目标描述
~140 kDa
外形
Purified antibody in 0.1M Tris-Glycine (pH7.4) 15mM NaCl with 0.05% NaN3.
Format: Purified
Protein A purified
储存及稳定性
Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Prior to removing the cap, gently centrifuge the vial and gently mix the solution.
分析说明
Control
Mouse brain
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
法律信息
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
生物来源 | rabbit |
质量水平 | 100 |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | polyclonal |
species reactivity | mouse |
manufacturer/tradename | Chemicon? |
technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable |
NCBI登记号 | NP_036433.2 |
UniProt登记号 | O88382 |
运输 | wet ice |
Gene Information | human ... MAGI2(9863) |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 1 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich