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Anti-NPTII antibody produced in rabbit

品牌
Sigma-Aldrich
货号
N6537
规格纯度
affinity isolated antibody, buffered aqueous solution
价格
7044.8 *本价格含增值税费
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半岛bd体育手机客户端 名称:
Anti-NPTII antibody produced in rabbit
Anti-Neomycin Phosphotransferase II
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

Neomycin phosphotransferase II (NPTII) gene also known as the kanamycin-resistance marker gene, encoding for NPTII (30 kDa) an aminoglycoside 3′ -phosphotransferase II (APHII), was isolated from theE. colitransposon Tn5.

特异性

The antibody recognizes bacterial NPTII expressed in transgenic tobacco plants.

免疫原

synthetic peptide corresponding to amino acids 133-150 located at the mid-region ofE. coliNPTII, conjugated to KLH.

应用

Anti-NPTII antibody has been used in immunoblotting.

生化/生理作用

The Neomycin phosphotransferase II (NPTII) gene confers resistance to some aminoglycoside antibiotics including neomycin and kanamycin in bacteria and plant cells and to G418 (Gibco Geneticin reagent) sulfate in mammalian cells. NPTII phosphorylates and inactivates kanamycin, preventing kanamycin from binding to the 30S ribosomal subunit to inhibit protein synthesis, thus rendering cells resistant to the antibiotic. NPTII serves as a marker for the selection of genetically engineered crops.

外形

0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

MDL编号 MFCD01633903
NACRES NA.41

半岛bd体育手机客户端 性质

生物来源 rabbit
质量水平 200
偶联物 unconjugated
抗体形式 affinity isolated antibody
antibody product type primary antibodies
克隆 polyclonal
形式 buffered aqueous solution
分子量 antigen 30 kDa
species reactivity plant
增强验证 recombinant expression
Learn more about Antibody Enhanced Validation
technique(s) western blot: 2-4 μg/mL using extract (cytosolic fraction) from the leaves of transgenicNicotiana tabacumexpressingE. coliNPTII
运输 dry ice
储存温度 ?20℃

安全信息

储存分类代码 12 - Non Combustible Liquids
WGK WGK 3
闪点(F) Not applicable
闪点(C) Not applicable

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