Papain Agarose from papaya latex

半岛bd体育手机客户端 说明

应用

Papain is used in dissecting solutions. It is used to produce Fab fragments of antibodies. It is used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues. Papain has been used to isolate morphologically intact cortical neurons from postnatal rats. Limited papain digestion is used for structural studies of enzymes and other proteins. In addition, papain is used in red cell serology to modify the red cell surface to enhance or destroy the reactivity of many red cell antigens. Papain is useful for platelet serology studies. Papain has also been used in the enzymatic synthesis of amino acids, peptides, and other molecules. Product P4406 is agarose-linked papain and is provided as a lyophilized powder stabilized with lactose. Product P4406 has been used to digest purified anti-JMJD6 hamster monoclonal antibody into Fab fragments.
Used to produce Fab fragments of antibodies. Also used for cell dissociation since it has been shown to be more effective and less damaging with certain tissues.

包装

25, 50, 100 units in glass bottle

生化/生理作用

Papain papaya latex has antifungal activity againstC. albicans. It is a cysteine protease that cleaves peptide bonds of basic amino acids, leucine, or glycine.
. The pH optimum is 6.0-7.0
. Papain hydrolyzes esters and amides. Papain consists of a single polypeptide chain with three disulfide bridges and a sulfhydryl group necessary for activity of the enzyme.
A cysteine protease that cleaves peptide bonds of basic amino acids, leucine or glycine. pH optimum 6.0-7.0
Also hydrolyzes esters and amides.

单位定义

One unit will hydrolyze 1.0 μmole of BAEE per min at pH 6.2 at 25℃, unless otherwise indicated below.

外形

Lyophilized powder, stabilized with lactose

基本信息

MDL编号	MFCD00131791
NACRES	NA.54

半岛bd体育手机客户端 性质

形式	lyophilized powder
质量水平	200
specific activity	90-150 units/mL packed gel
分子量	21 kDa
标记范围	3,000-5,000 units per g agarose (at pH7.0 at 30 ℃.)
基质	beaded agarose
储存温度	?20℃

安全信息

象形图
警示用语：	Danger
危险声明	H317 - H334
预防措施声明	P261 - P280 - P342 + P311
危险分类	Resp. Sens. 1 - Skin Sens. 1
储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	dust mask type N95 (US), Eyeshields, Faceshields, Gloves