Cholesterol Esterase from bovine pancreas

半岛bd体育手机客户端 说明

一般描述

Cholesterol Esterase from bovine pancreas is a member of α-β hydrolase superfamily. The core of the protein comprises 11 β-strands, surrounded by 15 α-strands. Catalytic triad is in the centre of the molecule. The C-terminus possesses hydrophobic residues.

应用

Cholesterol Esterase from bovine pancreas has been used:

• in γ-oryzanol-hydrolyzing activity
• in cholesterol esterase enzyme activity for quantification of cholesterol and cholesterol esters associated in tear film and lenses samples
• to testin vitrodegradation of poly(fumaroyl bioxirane) maleate (PFM) polymer designed for bone tissue engineering

Cholesterol esterase bound to membrane-associated heparin on brush border membranes aids in the transport of cholesterol and free fatty acid across the membrane. This enzyme is widely used in the determination of serum cholesterol in clinical laboratories. The enzyme from Sigma has been used to evaluate the inhibitory and antioxidant functions of the methanol extract of the Camellia sinensisleaves under in vitroconditions. The enzyme has also been used to digest human serum samples to confirm the presence and position of acyl esters of 7α-hydroxycholesterol.
Enzyme responsible for the hydrolysis of many of the fatty acid esters of cholesterol.
Optimum pH range: 6-8
Activators: Bisphenol A diglycidyl ether, cAMP-dependent protein kinase type II, ethanol, methanol, n-butanol, phosphatidylcholine, phosphatidylethanolamine, sodium taurocholic acid
Inhibitors: Bisphenol A methacrylate, diisopropylfluorophosphate, enolase, Hg ²⁺, sodium fluoride, phosphatidic acid, phosphatidylcholine, phosphatidylserine

包装

100, 500 units in poly bottle

生化/生理作用

Cholesterol esterase (CE) is a reversible enzyme that can hydrolyze or synthesize fatty acid esters of cholesterol and other sterols. Hydrolysis of water insoluble long chain fatty acid esters requires bile salt activation. Hydrolysis of water soluble esters of short chain fatty acids and lysophospholipids does not require activation by bile salts. It also hydrolyzes tri-, di-, and mono-acylglycerols, phospholipids, lysophospholipids, and ceramide. This monomeric glycoprotein may have multiple functions in lipid and lipoprotein metabolism, as well as in atherosclerosis. Its molecular mass is found to be 84 kDa.

其他说明

部分纯化

单位定义

One unit will hydrolyze 1.0 μmole of cholesteryl oleate to cholesterol and oleic acid per minute at pH 7.0 at 37 ℃ in the presence of taurocholate.

外形

This product is partially purified from bovine pancreas and is supplied as a white to light brown lyophilized powder containing ≥20% protein (biuret) and potassium phosphate.

制备说明

Cholesterol esterase is soluble in 0.4 M potassium phosphate, pH 7.0 at 1 mg/mL concentration.

分析说明

Protein determined by biuret.

基本信息

酶学委员会(EC)编号	3.1.1.13 ( BRENDA | IUBMB )
EC 号	232-808-6
MDL编号	MFCD00071052

半岛bd体育手机客户端 性质

形式	lyophilized powder
质量水平	200
specific activity	≥200 units/g protein
分子量	84 kDa by SDS-PAGE
组成	protein, ≥20%
储存温度	?20℃

安全信息

储存分类代码	13 - Non Combustible Solids
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)