Lipopolysaccharides (rough strains) fromEscherichia coliF583 (Rd mutant)

半岛bd体育手机客户端 说明

一般描述

This product is phenol:chloroform:petroleum ether extracted from E. coli F 583 (Rd mutant). The source strain is from a private collection.

包装

5, 10 mg in glass bottle

应用

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

生化/生理作用

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

制备说明

Prepared by phenol-chloroform-petroleum ether extraction.

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80^oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

基本信息

EC 号	297-473-0
MDL编号	MFCD00164401
NACRES	NA.25

半岛bd体育手机客户端 性质

质量水平	200
生物来源	Escherichia coli(F583 Rd mutant)
形式	lyophilized powder
杂质	<3% Protein (Lowry)
运输	ambient
储存温度	2-8℃

安全信息

象形图
警示用语：	Danger
危险声明	H300
预防措施声明	P264 - P270 - P301 + P310 - P405 - P501
危险分类	Acute Tox. 2 Oral
储存分类代码	6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Gloves, type N95 (US)