半岛bd体育手机客户端 说明
一般描述
A cell-permeable compound that acts as a pan PAD inhibitor (IC50= 0.8 ?M, 6.2 ?M and 5.9 ?M for PAD1, PAD3, and PAD4, respectively) in enzymatic assays. It preferentially inactivates the calcium bound form of PAD4 in an irreversible manner, and is shown to complete with BAEE for the active site residue of this enzyme. A small but significant reduction in the efficiency of p300GDB-GRIP1 interaction is observed in transiently transfected CV-1 cells treated with Cl-amidine at concentrations between 25 ?M and 200 ?M,in vivo. Furthermore, this compound (200 ?M) together with HDAC inhibitor SAHA (0.4 ?M) has shown additive effects in inducing p21, GADD45, and PUMA expression and inhibiting cancer cell growth in a p53-dependent manner in U2OS cells.
包装
10 mg in Glass bottle
警告
Toxicity: Regulatory Review (Z)
重悬
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
其他说明
Knuckley, B., et al. 2010.Biochemistry23,4852.
Li, P., et al. 2010.Oncogene21,3153.
Luo, Y., et al. 2006.Biochemistry39,11727.
法律信息
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
基本信息
| 经验(实验)分子式 | C16H20ClF3N4O4 |
| 分子量 | 424.80 |
| NACRES | NA.77 |
半岛bd体育手机客户端 性质
| 质量水平 | 200 |
| 测定 | >95% (HPLC) |
| 形式 | solid |
| manufacturer/tradename | Calbiochem? |
| 储存条件 | OK to freeze |
| 颜色 | white |
| 溶解性 | DMSO: 50 mg/mL |
| 运输 | ambient |
| 储存温度 | ?20℃ |
安全信息
| 储存分类代码 | 11 - Combustible Solids |
| WGK | WGK 3 |
| 闪点(F) | Not applicable |
| 闪点(C) | Not applicable |
Sigma-Aldrich
m.cnreagent.com
