TBBz

半岛bd体育手机客户端 说明

应用

TBBz has been used as a CK2 inhibitor in HeLa cells and rat septal neurons.

包装

5, 25 mg in glass bottle

生化/生理作用

TBBz is a cell-permeable Casein Kinase-2 (CK2) inhibitor. CK2 inhibitors, 4,5,6,7-tetrabromobenzotriazole (TBBt, Sigma Cat. # T0826) and rabromobenzimidazole (TBBz), the latter of which was shown to discriminate between different molecular forms of CK2 in yeast. TBBt, with a pK(a) ~5, exists in solution at physiological pH almost exclusively (>99%) as the monoanion; whereas TBBz, with a pKa ~9, is predominantly (>95%) in the neutral form, both of obvious relevance to their modes of binding.In vitro,TBBt inhibits different forms of CK2 with Ki values ranging from 80 to 210 nM. TBBz discriminates better between CK2 forms, with Ki values ranging from 70-510 nM. TBBz is more effective than TBBt in inducing apoptosis and to a lesser degree, necrosis in transformed human cell lines. Dvelopment of shRNA strategies for the selective knockdown of the CK2α and CK2α′ isoforms reinforces the foregoing results, indicating that inhibition of CK2 leads to attenuation of proliferation.

制备说明

TBBz is soluble in DMSO (60 deg C) at a concentration that is greater than 10 mg/ml.

基本信息

经验(实验)分子式	C7H2N2Br4
分子量	433.72
MDL编号	MFCD04116202
PubChem化学物质编号	329826855
NACRES	NA.77

半岛bd体育手机客户端 性质

质量水平	100
测定	≥98% (HPLC)
形式	powder
溶解性	DMSO: >10 mg/mL at 60 ℃, clear
储存温度	2-8℃
SMILES string	Brc1c(Br)c(Br)c2[nH]cnc2c1Br
InChI	1S/C7H2Br4N2/c8-2-3(9)5(11)7-6(4(2)10)12-1-13-7/h1H,(H,12,13)
InChI key	LOEIRDBRYBHAJB-UHFFFAOYSA-N

安全信息

储存分类代码	6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects
WGK	WGK 3
闪点(F)	Not applicable
闪点(C)	Not applicable
个人防护装备	Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges