Carbonic Anhydrase II bovine

半岛bd体育手机客户端 说明

应用

Carbonic Anhydrase II bovine has been used as a control in native gel electrophoresis and X-ray absorption spectroscopy characterization ofThalassiosira weissflogiicarbonic anhydrase 1.
Carbonic anhydrase is used to create carbon dioxide capture systems and to research various purification techniques . Carbonic anhydrase is also used to study acid-base regulation in fish and carbonic anhydrase type II deficiency syndrome . Product C7749, Bovine carbonic anhydrase II (CA II), has been widely used as a model protein in the investigation of the protein folding process.

生化/生理作用

Carbonic Anhydrase is a zinc-containing enzyme that catalyzes the reversible conversion of carbon dioxide to bicarbonate. One of its main physiological roles is to maintain the acid-base balance in blood and other tissues. Lack of carbonic anhydrase results in carbonic anhydrase type II deficiency syndrome, which is an autosomal recessive disease that causes osteopetrosis, renal tubular acidosis and brain calcifications . Carbonic anhydrases are metalloenzymes since most active sites contain zinc. Carbonic anhydrases are widely distributed in plant and animal tissues where they are involved in diverse physiological processes, such as photosynthesis, pH homeostasis, calcification, and bone resorption. The enzymes can be localized in the cytosol or mitochondria, be membrane bound with extracellular domains, or be secreted. Bovine carbonic anhydrase II (CA II) is a cytosolic, single-chain, approximately 29 kDa enzyme.

单位定义

One unit will decrease th pH of a 20 mM tris buffer from pH 8.3 to 6.3 per minute at 0 ℃.

外形

Supplied as a solution in 20 mM Tris, pH 7.6, with 150 mM NaCl.

基本信息

酶学委员会(EC)编号	4.2.1.1 ( BRENDA | IUBMB )
NACRES	NA.54

半岛bd体育手机客户端 性质

质量水平	200
重组	expressed inE. coli
测定	≥90% (SDS-PAGE)
形式	buffered aqueous solution
specific activity	≥5,000 units/mg protein
分子量	~29 kDa
运输	wet ice
储存温度	?20℃

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable