HL60 Ast.3

半岛bd体育手机客户端 说明

细胞系来源

Human Caucasian promyelocytic leukaemia

细胞系描述

Variant of the promyelocytic cell line HL60 isolated after treating cells with α-particle radiation and then cloned in medium containing 1.25% DMSO. Cells are capable of differentiating into neutrophils by induction with 1.75% DMSO.

应用

Differentiation studies

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 14
D13S317: 8,11
D16S539: 11
D5S818: 12
D7S820: 11,12
THO1: 7
TPOX: 8,11
vWA: 16

培养基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).

传代培养常规

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37℃. Do not allow the culture medium to become acidic or the cell line will differentiate.

其他说明

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半岛bd体育手机客户端 性质

生物来源	human blood
生长模式	Suspension
核型	Modal no. = 93, Hypertetraploid
形态学	Lymphoblastoid
半岛bd体育手机客户端	Not specified
受体	Not specified
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice
储存温度	?196℃