半岛bd体育手机客户端 说明

细胞系来源

Human Burkitt′s lymphoma

细胞系描述

Clone of RAJI selected for adherence to glass or plastic. Fibroblast like cell line. RAJI ′A′ was developed from the adherent sub-population of RAJI cells. Karyotype analysis demonstrated that RAJI ′A′ contains typical human chromosomes and the modal total chromosome number (47-48) is the same as that of RAJI. RAJI ′A′ is also identical to RAJI with respect to isoenzyme composition, susceptibility to EBV infection and inducibility of latent EBV. Ethnicity: Black.

应用

Tumourigenicity studies

DNA图谱分析

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 13
D16S539: 8,11
D5S818: 10,13
D7S820: 10
THO1: 6,7
TPOX: 8,13
vWA: 17,19

培养基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm²using 0.25% trypsin/EDTA; 5% CO₂; 37℃.

其他说明

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半岛bd体育手机客户端 性质

生物来源	human lymph
生长模式	Suspension
核型	Modal no. 47-48
形态学	Fibroblast-like
半岛bd体育手机客户端	Not specified
受体	Not specified
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice
储存温度	?196℃