KB (HeLa derivative)

半岛bd体育手机客户端 说明

细胞系来源

Human cervix carcinoma

细胞系描述

Originally derived from an epidermoid carcinoma of the mouth of an adult male Caucasian. It was one of the early attempts to isolate and serially propagate a human cell line directly on glass as a monolayer. NB: This cell line contains HeLa marker chromosomes and expresses type A G6PD. For further information see Nature 1976;259:211; In Vitro 1978;14:469. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. Ethnicity: Black.

应用

KB has been used extensively in studies of cell nutrition and metabolism, cancer chemotherapy screening, tumourigenicity and viruses e.g. susceptible to Polio virus type I and Adenovirus type 3.

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm²using 0.25% trypsin/EDTA; 5% CO₂; 37℃.

其他说明

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半岛bd体育手机客户端 性质

生物来源	human cervix
生长模式	Adherent
核型	2n = 46
形态学	Epithelial-like
半岛bd体育手机客户端	Not specified
受体	Not specified
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice
储存温度	?196℃