JY human cell line

半岛bd体育手机客户端 说明

一般描述

Homozygous: No
Consanguineous: No

细胞系描述

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA图谱分析

A: 201
Cw: 702
B: 702
HLA DQA1: 3011
HLA DPB1: 201

血清学分析

HLA A: 2
HLA C: 7
HLA B: 7
Bw4/bw6: 6
HLA DR: 4 13
HLA DR52: 52
HLA DR53: 53
HLA DQ: 1 3

培养基

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

传代培养常规

When resuscitating from frozen, cells should be seeded at 0.5 x 10⁶cells/ml in the culture medium. The culture should establish log phase growth after 3-5 days. When growing well dilute every 1-3 days to 3 x 10⁵cells/ml. Maintain cultures between 3-9 x 10⁵cells/ml for maximum growth; 5% CO₂; 37℃. Cells form floating aggregates of varying size.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免责声明

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.

半岛bd体育手机客户端 性质

生物来源	human blood
种族	Amish
性别	male
生长模式	Suspension
核型	Not specified
形态学	Lymphoblastoid
technique(s)	cell culture | mammalian: suitable
运输	dry ice