半岛bd体育手机客户端 说明
细胞系来源
Human Caucasian B-prolymphocytic leukaemia, EBV transformed
细胞系描述
Derived from the peripheral blood of a human Caucasian male patient with prolymphocytic leukaemia. Cells have been subsequently transformed by treatment with EBV and TPA (12-0-tetradecanoylphorbol-13-acetate). JVM-13 cells express surface-membrane and cytoplasmic lambda light chain immunoglobulin. Immunological markers detected were HLA-DR, CD19, CD20, CD21, FMC7, CD38, CD71 and CD9.
应用
Study of B cell leukaemia
DNA图谱分析
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 9,11
D16S539: 11
D5S818: 11
D7S820: 9
THO1: 6,7
TPOX: 10,11
vWA: 16,18
培养基
RPMI 1640 + 4mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37℃.
其他说明
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半岛bd体育手机客户端 性质
生物来源 | human blood |
生长模式 | Suspension |
核型 | 46XY,+t(8,?13)(p12-21,?q13)+(8,?3)(p12-21,?q21)+I |
形态学 | Lymphoblastoid |
半岛bd体育手机客户端 | Not specified |
受体 | Not specified |
technique(s) | cell culture | mammalian: suitable |
相关疾病 | cancer |
运输 | dry ice |
储存温度 | ?196℃ |
Sigma-Aldrich