JEG3

半岛bd体育手机客户端 说明

细胞系来源

Human choriocarcinoma

细胞系描述

One of 6 clones derived from cells implanted into a hamster cheek pouch and then propagated on irradiated feeder layers of human fibroblasts. Cell line releases chorionic gonadotrophin and somatomammotrophin and progesterone. It is also able to transform steroid precursors to oestrone and oestradiol. JEG3 has the same DNA profile as BeWo (Sigma Catalogue number 86082803); it was established by serial cloning of BeWo .

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3x10,000 cells/cm²using 0.25% trypsin, 5% CO₂; 37℃. Vacuolisation will occur at confluency.

其他说明

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半岛bd体育手机客户端 性质

生物来源	human placenta
生长模式	Adherent
核型	modal no. 71
形态学	Epithelial
半岛bd体育手机客户端	Human chorionic gonadotrophin (hCG), somatomammotrophin
受体	Not specified
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice
储存温度	?196℃