G-361

半岛bd体育手机客户端 说明

细胞系来源

Human Caucasian malignant melanoma

细胞系描述

Established from a malignant melanoma of a 31 year old male Caucasian. The cells produce melanin for up to 50 population doublings. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

应用

Hormone studies

DNA图谱分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11
D16S539: 15
D5S818: 11
D7S820: 8,11
THO1: 7,9
TPOX: 8,10
vWA: 15,17

培养基

McCoy′s 5a + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm²using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37℃. Cells are slow in attaching.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

半岛bd体育手机客户端 性质

生物来源	human skin
生长模式	Adherent
核型	Triploid, modal n.o 69
形态学	Epithelial
半岛bd体育手机客户端	Melanin
受体	Not specified
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice
储存温度	?196℃