C99

半岛bd体育手机客户端 说明

细胞系来源

Human colorectal adenocarcinoma, moderately well differentiated, Dukes′ stage C

细胞系描述

The C99 cell line was established from a 69-year old male patient with a moderately well differentiated adenocarcinoma of the rectum classified as Dukes′ stage C.

DNA图谱分析

STR-PCR Data:
Amelogenin: X
CSF1PO: 10,11
D13S317: 8,14
D16S539: 11,13
D5S818: 11,12
D7S820: 9,11
THO1: 8,9.3
TPOX: 8,10
vWA: 16,17

培养基

Iscove′s Modified Dulbecco′s Medium, + 10% Foetal Bovine Serum (FBS) + 2 mM Glutamine

传代培养常规

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm? using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37 ℃. C99 cells grow very slowly (see growth curve); following resuscitation or subculture the cells take at least 48 hours

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免责声明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.

半岛bd体育手机客户端 性质

生物来源	human colon
生长模式	Adherent
核型	Not specified
形态学	Epithelial
半岛bd体育手机客户端	Not specified
受体	Not specified
technique(s)	cell culture | mammalian: suitable
相关疾病	cancer
运输	dry ice