Anti-PP2A Antibody, C subunit, clone 1D6

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一般描述

Type 2 protein serine/threonine phosphatase (PP2A) is a trimer composed of a catalytic C subunit and two regulatory (A and B) subunits; many isoforms for the A and B subunits have been found. PP2A has a high specific activity against the α subunit of phosphorylase kinase and is insensitive to Inhibitor-2. PP2A is distinguishable from PP2B/Calcineurin and PP2C by its partial activity in the absence of divalent cations, and by its high sensitivity to inhibition by okadaic acid. It is also thought to be the principal downmodulator of serine/threonine kinase cascades. PP2B requires Ca2+/calmodulin for activity, and is an activator of NFAT transcription factors. PP2C requires Mg2+, but is insensitive to okadaic acid, microcystin LR, or the calmodulin inhibitor trifluoperazine.

特异性

This antibody recognizes PP2A catalytic subunit, Mr 36 kDa.

免疫原

A 16 residue synthetic peptide (C-RGEPHVTRRTPDYFL) corresponding to amino acids 295-309 of the 36 kDa catalytic subunit of human protein phosphatase 2A (PP2A). Clone 1D6.
Epitope: a.a. 295-309

应用

Detect PP2A using this Anti-PP2A Antibody, C subunit, clone 1D6 validated for use in IC, IP & WB.
Research Sub Category
Neurotransmitters & Receptors

Neurodegenerative Diseases
Research Category
Signaling

Neuroscience
Immunoprecipitation:
4 ?g of a previous lot immunoprecipitated PP2A from 500 ?g of A431 RIPA lysate. Note: This antibody has been reported not to block phosphatase activity.

Immunocytochemistry:
5-10 ?g/mL of a previous lot of this antibody showed positive immunostaining for PP2A in A431 cells fixed for 10 minutes with 4% paraformaldehyde in PBS.

质量

Routinely evaluated by western blot on human A431 or murine 3T3/NIH RIPA cell lysates.

Western Blot Analysis:
0.5-2 ?g/mL of this lot detected PP2A in human A431 and previously in murine 3T3 RIPA cell lysates.

目标描述

36 kDa

外形

Purified mouse monoclonal IgG2bκ in buffer containing 0.1M Tris-Glycine, pH 7.4, 0.15 M, NaCl, and 0.05% sodium azide. Frozen solution.
Protein A chromatography
Format: Purified

储存及稳定性

Stable for 1 year at -20?C from date of receipt. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

分析说明

Control
HeLa whole cell lysate, mouse NIH3T3 cells, A431 cell lysate.
Included Positive Antigen Control: Catalog # 12-301, non stimulated A431 lysate. Add 2.5 μL of 2-mercaptoethanol per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced lysate per lane for minigels.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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