BF-2

半岛bd体育手机客户端 说明

细胞系来源

Fish Bluegill fry caudal trunk

细胞系描述

Derived from a trypsinised suspension of pooled caudal portions of the trunk of one year old fingerlings. Will grow at temperatures from 15℃ to 33℃. Cells susceptible to lymphocystis virus of fish and tadpole edema virus (TEV) of frogs.

应用

Virus studies, targets for bacterial cytotoxins

培养基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS) (pH7.4 - 7.5).

传代培养常规

Split sub-confluent cultures (70-80%) 1:2 to 1:4 using 0.25% trypsin/EDTA; 5% CO₂; 23℃. A replication capacity of up to 50 passages is expected. Fish cell lines detach easily during transit if the culture is too young. After resuscitation, split cells

其他说明

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半岛bd体育手机客户端 性质

生物来源	fish trunk
生长模式	Adherent
核型	2n = 48
形态学	Fibroblast
半岛bd体育手机客户端	Not specified
受体	Not specified
technique(s)	cell culture | mammalian: suitable
运输	dry ice
储存温度	?196℃