SV40 T-antigen from simian virus

半岛bd体育手机客户端 说明

生化/生理作用

The large T antigen (TAg) of Simian virus 40 acts as an initiator of DNA replication, participates in cellular transformation, and induces cell growth. A number of functional domains have been characterized including the N-terminal J domain, nuclear localization signal (NLS), internal helicase domain, Rb and topoisomerase I interacting domain, p53 and ATP binding domain. The p53-interacting domain is also essential for binding p300/CBP. Phosphorylation of TAg by several kinases may regulate its function both positively and negatively.
The viral replication of simian virus 40 (SV40) is carried out by a single protein, T-antigen. This feature makes the virus a useful system for understanding the process of replication and to identify the components of replication machinery. It functions as a DNA helicase along with a cellular single-strand DNA binding protein and topoisomerase I or II, leading to extensive origin unwinding and formation of a pre-initiation complex. The T-antigen associates with two tumor suppressor proteins, p53 and the retinoblastoma protein pRb, and inactivates them.

外形

Clear and colorless frozen liquid solution

制备说明

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

半岛bd体育手机客户端 性质

生物来源	simian virus
重组	expressed in insect cells
测定	≥80% (SDS-PAGE)
形式	frozen liquid
分子量	~83.3 kDa
包装	pkg of 5 μg
储存条件	avoid repeated freeze/thaw cycles
浓度	400 μg/mL
颜色	clear colorless
NCBI登记号	AAG39214.1
UniProt登记号	P03070
运输	dry ice
储存温度	?70℃
Gene Information	simian virus ... SV40gp6(1489531)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable