半岛bd体育手机客户端 说明
一般描述
Nanodisc technology is an approach to render membrane proteins soluble in aqueous solutions in a native-like bilayer environment, where the membrane proteins remain stable and active. The Nanodisc concept is derived from high density lipoprotein (HDL) particles and their primary protein component, apolipoprotein. The Nanodisc is a non-covalent structure of phospholipid bilayer and membrane scaffold protein (MSP), a genetically engineered protein, which mimics the function of Apolipoprotein A-1 (ApoA-1).
The first MSP, MSP1, was engineered with its sequence based on the sequence of A-1, but without the globular N-terminal domain of native A-1. The MSP1E1D1 variant of MSP1 deletes the first 11 amino acids in the Helix 1 portion (referred to as “H0.5” in the acccompanying figure) of the original MSP1 sequence (which is known separately as MSP1D1), and also repeats the Helix 4 sequence of the original MSP1 sequence between the parent Helix 4 and Helix 5 segments of MSP1D1.
应用
For guidelines on the use of this and other MSP′s to prepare Nanodiscs, please visit our Protocols for Membrane Scaffold Proteins and Nanodisc Formation page.
法律信息
Nanodisc technology, and many of its uses, are covered by the following patents held by the University of Illinois.
基本信息
NACRES | NA.26 |
半岛bd体育手机客户端 性质
质量水平 | 200 |
重组 | expressed inE. coli |
测定 | ≥90% (SDS-GE) |
形式 | buffered aqueous solution |
运输 | ambient |
储存温度 | ?20℃ |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich