Anti-Y14 Antibody, clone 4C4

半岛bd体育手机客户端 说明

一般描述

Y14 (RNA binding motif protein 8A or RBM8A), an RNA-binding protein, contains a conserved RNA-binding motif. Y14 is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs. It is thought that the protein remains associated with spliced mRNAs as a tag to indicate where introns had been present, thus coupling pre- and post-mRNA splicing events. Previously, it was thought that two genes encode this protein, RBM8A and RBM8B; it is now thought that the RBM8B locus is a pseudogene. Two alternative start codons result in two forms of the protein, and this gene also uses multiple polyadenylation sites.

特异性

This antibody recognizes Y14.

免疫原

Epitope: Unknown
Purified human Y14.

应用

Anti-Y14 Antibody, clone 4C4 is a Mouse Monoclonal Antibody for detection of Y14 also known as Binder of OVCA1-1, RNA binding motif protein 8A, Ribonucleoprotein RBM8A & has been validated in WB, ICC & IP.
Research Sub Category
RNA Binding Protein (RBP)
Immunocytochemistry Analysis: 1:500 dilution from a representative lot detected Y14 in HeLa and NIH/3T3 cells.
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in immunoprecipitation. Kashima, I., et al. (2006). Genes & Dev. 20:355-367.
Research Category
Epigenetics & Nuclear Function

质量

Evaluated by Western Blot in HeLa cell lysate.
Western Blot Analysis: 0.5 ?g/ml of this antibody detected Y14 on 10 ?g of HeLa cell lysate.

目标描述

24 kDa

外形

Purified mouse monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8°C from date of receipt.

分析说明

Control
HeLa cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	4C4, monoclonal
species reactivity	mouse, rat, human
technique(s)	immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable
同位素/亚型	IgG2bκ
NCBI登记号	NP_005096.1
UniProt登记号	Q9Y5S9
运输	wet ice
Gene Information	human ... RBM8A(9939)

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable