半岛bd体育手机客户端 说明
一般描述
Paxillin is involved in focal adhesion formation during cell adhesion and migration. Paxillin contains LD motifs, LIM domains, and an SH3- and SH2-binding domain that participate in a variety of protein-protein interactions with kinases, GTPase-activating proteins, and cytoskeletal proteins. Phosphorylation of paxillin occurs at both tyrosine and serine sites. Tyrosine phosphorylation of paxillin occurs in response to growth factors, neuropeptides, and integrins. The major sites of tyrosine phosphorylation include Tyr31 and Tyr118. Both of these sites may be involved in Crk binding to paxillin during integrin-mediated cell adhesion. These sites may provide docking motifs for recruitment of other signaling molecules to focal adhesions.
特异性
The antibody detects Paxillin phosphorylated at Tyr31.
免疫原
Clone M102 was generated from phospho-Paxillin (Tyr31) synthetic peptide (coupled to KLH) corresponding to amino acid residues around tyrosine 31 of human paxillin. This human sequence is highly conserved in rat and mouse paxillin.
应用
Research Category
Cell Structure
Research Sub Category
Cytoskeletal Signaling
Detect phospho-Paxillin (Tyr31) using this Anti-phospho-Paxillin (Tyr31) Antibody, clone M102 validated for use in WB.
质量
Evaluated by western Blot in pervanadate treated L6 cell lysates.
Western Blot Analysis: 1:500 dilution of this lot detected phospho-Paxillin on 10 ?g of pervanadate treated L6 lysates.
目标描述
72 kDa
联系
Replaces: MAB1146
外形
Purified monoclonal IgG1 in phosphate-buffered saline, 50% glycerol, 1 mg/mL BSA, and 0.05% sodium azide.
Protein A chromatography
Format: Purified
储存及稳定性
Maintain at -20°C for one year from date of receipt. Do not aliquot.
Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.
分析说明
Control
Pervanadate treated L6 cell lysates
其他说明
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified antibody |
antibody product type | primary antibodies |
克隆 | M102, monoclonal |
species reactivity | human |
technique(s) | western blot: suitable |
同位素/亚型 | IgG1 |
UniProt登记号 | P49023 |
运输 | wet ice |
安全信息
储存分类代码 | 12 - Non Combustible Liquids |
WGK | WGK 2 |
闪点(F) | Not applicable |
闪点(C) | Not applicable |
Sigma-Aldrich