Anti-p21/WAF1/Cip1 Antibody

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一般描述

Two families of cyclin dependent kinase inhibitors (CKIs) have been identified. The p21WAF1/Cip1 family inhibits all kinases involved in the G1/S transition. The p16INK4a family inhibits Cdk4 and Cdk6 specifically. The biochemical activities and patterns of expression of CKIs during development implicate these proteins as the primary effectors of signaling pathways that control cell cycle exit, an event that is critical for differentiation. Studies have shown that p21WAF1/Cip1 binds tightly to the G1 and S phase kinases, cyclin E/Cdk2, cyclin D/Cdk4, and cyclin A/Cdk2, and effectively inhibits their activity, whereas p21WAF1/Cip1 is a relatively poor inhibitor of the G2/M phase kinase cyclin B/Cdc2. In addition, p21WAF1/Cip1 inhibits proliferating cell nuclear antigen (PCNA), binds to and inhibits inactivation of Rb, which is essential for cell cycle progression, and may also interact with E2F and protect cells against p53-mediated apoptosis.

特异性

Recognizes Cip1, MW 21 kDa
Other species cross-reactivity is unknown.

免疫原

Epitope: amino acids 1-80
Recombinant full-length human Cip1 with a histidine tag.

应用

Research Sub Category
Cell Cycle, DNA Replication & Repair
Use Anti-p21/WAF1/Cip1 Antibody (Mouse Monoclonal Antibody) validated in WB, IP to detect p21/WAF1/Cip1 also known as CDK-interacting protein or cyclin-dependent kinase inhibitor 1A.
Research Category
Epigenetics & Nuclear Function
Immunoprecipitation: 4 μg of a previous lot immunoprecipitated Cip1 from 500 μg of HeLa nuclear extract.

质量

Evaluated by western blotting on HeLa nuclear extract.
Western Blot Analysis: 0.5-2 μg/mL of this antibody detected Cip1 in 20 μg of HeLa nuclear extract

目标描述

21 kDa

外形

Purified, pooled monoclonal mouse IgGs in buffer containing 0.1 M Trisglycine, pH 7.4, 0.15 M NaCl containing 0.05% sodium azide. Frozen solution.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at from date of receipt.

分析说明

Control
Included Positive Antigen: Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 5-10 μL of lysate and boil for 5 minutes to reduce the preparation. Use 20 μg per lane for minigels.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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