Anti-mTOR Antibody, clone 21D8.2

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一般描述

mTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and G (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.

特异性

This antibody recognizes mTOR.

免疫原

Flag-tagged full-length mTOR protein

应用

Anti-mTOR Antibody, clone 21D8.2 is an antibody against mTOR for use in WB & IC.
Research Category
Signaling
Research Sub Category
PI3K, Akt, & mTOR Signaling
Immunocytochemistry Analysis: A 1:500 dilution of this antibody from a previous lot detected mTOR in C2C12, HeLa, and NIH/3T3 cell lysates.

质量

Evaluated by Western Blot in HEK293 cell lysate.
Western Blot Analysis: 0.5 ?g/mL of this antibody detected mTOR on HEK293 cell lysate.

目标描述

289 kDa

联系

Replaces: 04-385

外形

Format: Purified
Purified immunoglobulin containing 0.1 M Tris-Glycine, pH 7.4, 150 mM NaCl and 0.05% Sodium Azide.
Protein G Purified

储存及稳定性

Maintain refrigerated at 2-8°C for 1 year from date of receipt.

分析说明

Control
Hek293 cell lysate

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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