Anti-LSD1/BHC110 Antibody

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一般描述

Histone demethylase that specifically demethylates ′Lys-4′ of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Demethylates both mono- and tri-methylted ′Lys-4′ of histone H3. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 ′Lys-4′ on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. May also demethylate ′Lys-9′ of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes.

特异性

Recognizes LSD1, Mr 110 kDa.

免疫原

GST fusion protein corresponding to residues 23-138 of human LSD1.

应用

Anti-LSD1/BHC110 Antibody is a Mouse Monoclonal Antibody for detection of LSD1/BHC110 also known as BRAF35-HDAC complex protein BHC110, lysine (K)-specific demethylase 1 & has been validated in ChIP, IF, IHC, IP & WB.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Histones

Chromatin Biology
Chromatin Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate LSD1 from chromatin.
Immunoprecipitation:
Recommended.
Immunohistochemistry:
This antibody has been reported by an independent laboratory to detect LSD1 using paraffin-embedded tissues. (Metzger, E., 2005)
Immunofluorescence:
Recommended.

质量

Routinely evaluated by western blot.
Western Blot Analysis:
1:500-1:2000 dilution of this lot detected LSD1 in RIPA lysates from HeLa cells.

目标描述

110 kDa

联系

Replaces: CBL770

外形

Unpurified
Mouse monoclonal in buffer containing 0.05% sodium azide and 30% glycerol.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

分析说明

Control
HeLa cell lysate.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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