Anti-FOXA1 Antibody, clone 2F83

半岛bd体育手机客户端 说明

一般描述

FOXA1 (HNF3A, Hepatocyte nuclear factor 3-alpha) is member of the Forkhead family of winged-helix transcription factors. It is known to be involved in the development and differentiation of several organs including liver, kidney, pancreas, lung, prostate and mammary gland (Lupien, 2008). High expression of FOXA1 is often seen in tumors arising from both the prostate and breast where it is believed to interact with either the androgen receptor or estrogen receptor α, in each of the tumor type respectively (Lupien, 2008). In the case of ER?, FOXA1 has gained notice because of its interactions with the cis-regulatory regions in heterochromatin and because it enhances the interaction of ER? with chromatin (Badve, 2007).

特异性

This antibody detects human FOXA1 (HNF3α).
Other species have not been tested.

免疫原

Recombinant human GST-FOXA1 protein encompassing amino acids 7-86.
Epitope: N-terminus

应用

Research Sub Category
Transcription Factors
Immunohistochemisty:
Independent labs have verified this clone to work in paraffin-embedded IHC (IH(P)) of various mouse tissues including both embryonic and adult brain, adult digestive tract, adult respiratory tract, and adult urinary tract. Staining was performed using the antibody at a 1:2,000 dilution, but dilutions and conditions must be user optimized.
Research Category
Epigenetics & Nuclear Function
Use Anti-FOXA1 Antibody, clone 2F83 (Mouse Monoclonal Antibody) validated in WB, ICC, IHC(P) to detect FOXA1 also known as Forkhead box protein A1, hepatocyte nuclear factor 3 alpha.

质量

Evaluated by western blot on HeLa nuclear extracts.
Western Blot Analysis: 1:1,000 dilution of this antibody was used to detect FOXA1 in HeLa nuclear extract.

目标描述

49 kDa

外形

Format: Purified
Purified mouse monoclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Protein G Purified

储存及稳定性

Stable for 1 year at 2-8?C from date of receipt.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
克隆	2F83, monoclonal
species reactivity	human, mouse
technique(s)	immunocytochemistry: suitable immunohistochemistry: suitable (paraffin) western blot: suitable
同位素/亚型	IgG1κ
NCBI登记号	NP_937987
UniProt登记号	P55317
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable