半岛bd体育手机客户端 说明
一般描述
Epidermal growth factor receptor (UniProt: P00533; also known as EC:2.7.10.1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1) is encoded by the EGFR (also known as ERBB, ERBB1, HER1) gene (Gene ID: 1956) in human. EGFR is a single-pass type I membrane glycoprotein that is ubiquitously expressed. It is synthesized with a signal peptide (1-24), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 25-645), a transmembrane domain (aa 646-668), and a long cytoplasmic domain (669-1210). It possesses receptor tyrosine kinase activity and upon binding of ligands activates several signaling cascades. Ligand binding is known to trigger receptor homo- and/or heterodimerization, which stimulates its intrinsic intracellular protein-tyrosine kinase activity. As a result, autophosphorylation of tyrosine residues in the C-terminal domain of EGFR occurs, which results in activation of several downstream signaling cascades, including the Ras-RAF-MEK-ERK, PI3 kinase-Akt, PLCγ-PKC, and STAT. EGFR phosphorylation at serine 695 is reported to be only partial and occurs only if threonine 693 is phosphorylated. EGFR also undergoes mono and polyubiquitination upon EGF stimulation. However, this ubiquitination does not affect tyrosine kinase activity of the receptor or its signaling capacity. EGFR is commonly overexpressed and is mutated in many human malignancies and is often associated with aggressive phenotypes.
Reacts with external domain of EGF receptor on all human cells. Competes with EGF and TGF-α for binding on human cells.
特异性
Recognizes both phosphorylated and non-phosphorylated human EGF receptors.
免疫原
Human A431 membrane proteins.
应用
Detect EGFR using this Anti-EGFR Antibody, neutralizing, clone LA1 validated for use in Immuncytochemistry, Immunohistochemistry, Immunoprecipitation, Neutralization, and Western Blotting.
Immunocytochemistry (IC) Analysis: 2μg/mL of a representative lot detected EGFR in A431 cells.
Immunohistochemistry (Paraffin) (IHC) Analysis: A 1:50 dilution of this antibody detected EGFR in Human placenta tissue sections.
Immunoprecipitation Analysis: A representative lot of this antibody immunoprecipitated EGFR in Huh-7.5 cells. (Diao, J., et al. (2012). J. Virol. 86(20); 10935-10949).
Western Blotting Analysis: 1 mg/mL of a representative lot detected EGFR in A431 cell lysate.
Research Sub Category
Growth Factors & Receptors
Research Category
Signaling
质量
routinely evaluated on RIPA lysates from non-stimulated human A431 cells
目标描述
180 kDa
联系
Replaces: 04-337; 04-338
外形
Lyophilized protein G Purified mouse immunoglobulin. Lyophilized from 0.1M Tris-glycine, pH 7.4, 0.15M NaCl containing no preservatives.
Format: Purified
Protein G Purified
储存及稳定性
Lyophilized: 2 years at -20°C; Rehydrated: 1 month at 4°C or 6 months at -20°C
分析说明
Control
Positive Control Included: Non-Stim A431 Cell Lysate (12-301)
法律信息
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
基本信息
eCl@ss | 32160702 |
NACRES | NA.41 |
半岛bd体育手机客户端 性质
质量水平 | 100 |
生物来源 | mouse |
抗体形式 | purified immunoglobulin |
antibody product type | primary antibodies |
克隆 | LA1, monoclonal |
species reactivity | human |
manufacturer/tradename | Upstate? |
technique(s) | immunocytochemistry: suitable immunohistochemistry: suitable immunoprecipitation (IP): suitable neutralization: suitable western blot: suitable |
同位素/亚型 | IgG1 |
NCBI登记号 | NM_005228.3 NM_201282.1 NM_201283.1 NM_201284.1 |
UniProt登记号 | P00533 |
运输 | dry ice |
安全信息
储存分类代码 | 11 - Combustible Solids |
WGK | WGK 1 |
Sigma-Aldrich