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位置: 首页> 品牌> Sigma-Aldrich> Anti-Eck/EphA2 Antibody, clone D7

Anti-Eck/EphA2 Antibody, clone D7

品牌
Sigma-Aldrich
货号
05-480
规格纯度
clone D7, Upstate?, from mouse
价格
7365.52 *本价格含增值税费
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半岛bd体育手机客户端 名称:
Anti-Eck/EphA2 Antibody, clone D7
EPH receptor A2, Epithelial cell kinase, Tyrosine-protein kinase receptor ECK, ephrin receptor EphA2, epithelial cell receptor protein tyrosine kinase, protein tyrosine kinase, receptor protein tyrosine kinase regulated by p53 and E2F-1
半岛bd体育手机客户端 介绍:

半岛bd体育手机客户端 说明

一般描述

EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands.
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation.

特异性

This antibody recognizes Epithelial Cell Kinase (Eck/EphA2), Mr 140 kDa

免疫原

Native protein isolated by purification of phosphotyrosine-containing proteins. Clone D7.

应用

This Anti-Eck/EphA2 Antibody, clone D7 is validated for use in IC, IP, EA, WB for the detection of Eck/EphA2.
Research Category
Neuroscience
Protein Kinase Assay:
A previous lot of this antibody has been reported to have been used in an immunoprecipitation autophosphorylation assay, using a Mn-PIPES reaction buffer (Romer, L., 1994).
Immunoprecipitation:
A previous lot of this antibody has been reported to immunoprecipitate Eck from 500 ?g of a human breast epithelial cell line which had been lysed in TBS containing 1% Triton X-100. Use 1-4 ?g per reaction.
Immunocytochemistry:
A previous lot of this antibody has been reported to immunostain Eck in human, mouse and rat epithelial cells fixed with 3.7% formaldehyde solution and permeabilized with 0.5% Triton X-100 in TBS.
Research Sub Category
Growth Cones & Axon Guidance

质量

Routinely evaluated by immunoblot on RIPA lysates from human A431, foreskin fibroblasts, murine 3T3/A31 or rat L6 cells.
Western Blot Analysis:
0.5-2 ?g/mL of this lot detected Eck in RIPA lysates from human A431 and previously from foreskin fibroblasts, murine 3T3/A31 and rat L6 cells.

目标描述

140 kDa

外形

Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1 in buffer containing 0.2 M Tris-glycine, pH 7.4, 0.15 M NaCl, with 0.05% sodium azide and 30% glycerol.

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. NOTE: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

分析说明

Control
A431 cell lysate, CHO cell lysate, foreskin fibroblasts, 3T3/A31 cells, L6 cells.
Included Positive Antigen Control: Catalog # 12-301, non stimulated A431 lysate. Add 2.5 ?L of 2-mercaptoethanol/100 ?L of lysate and boil for 5 minutes to reduce the preparation. Load 20 ?g of reduced lysate per lane for minigels.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss 32160702
NACRES NA.41

半岛bd体育手机客户端 性质

质量水平 100
生物来源 mouse
抗体形式 purified immunoglobulin
antibody product type primary antibodies
克隆 D7, monoclonal
species reactivity bovine, human, rat, mouse, canine
manufacturer/tradename Upstate?
technique(s) activity assay: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
同位素/亚型 IgG1
NCBI登记号 NM_004431
UniProt登记号 P29317
运输 dry ice

安全信息

储存分类代码 10 - Combustible liquids
WGK WGK 1

Sigma-Aldrich

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