Anti-CFTR Antibody, clone M3A7

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一般描述

CFTR (Cystic Fibrosis Transmembrane Regulator) is an integral membrane protein shown to be involved in the transport of chloride ions. It is found on the surface of the epithelial cells lining the lungs and other organs. CFTR defects are the cause of cystic fibrosis. Cystic Fibrosis (CF) is a common lethal genetic disease caused by mutations of the gene coding for the cystic fibrosis transmembrane conductance factor, a cAMP regulated chloride channel. Approximately 70% of all CF cases share the deletion of a phenylalanine at position 508 (delta F508), which results in abnormal chloride transport. Since the CF mutation is lethal, most often by lung and liver disease, it raises the question of why this genetic disease remains as common as it is.

特异性

This antibody recognizes CFTR, Mr 170 kDa.
Other species not tested.

免疫原

GST-fusion protein corresponding to residues 1197-1480 of human cystic fibrosis transmembrane conductance regulator (CFTR). The epitope maps within amino acids 1365-1395. Clone M3A7.
Epitope: a.a. 1365-1395

应用

Research Category
Neuroscience
Immunoprecipitation:
This antibody has been reported to immunoprecipitate CFTR. (Kartner, N., 1998.)
Immunohistochemistry:
This antibody has been reported to immunostain CFTR in human pancreatic tissue sections. (Kartner, N., 1998.)
Research Sub Category
Ion Channels & Transporters
Detect CFTR using this Anti-CFTR Antibody, clone M3A7 validated for use in IH, IP & WB.

质量

Routinely evaluated by western blot on human T84 colon carcinoma epithelial RIPA cell lysates or from CFTR-transfected BHK2.
Western Blot Analysis:
0.5-2 ?g/mL of this lot detected CFTR from 20-50 ?g of human T84 colon carcinoma epithelial RIPA cell lysates. 0.5-2 ?g/mL of a previous lot detected CFTR from CFTR-transfected BHK (Haardt, M., 1999).
Note: Do not boil the lysate. Instead incubate at 37°C for 30 minutes. CFTR can run as a diffuse protein on SDS-PAGE.

目标描述

170 kDa

外形

Format: Purified
Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-glycine, pH 7.4, 0.15 M NaCl, 0.05% sodium azide and 30% glycerol.
Protein G Purified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol-containing solutions to become frozen during storage.

分析说明

Control
T84 cell lysate.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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