Anti-Axin-1 Antibody, clone A5

半岛bd体育手机客户端 说明

一般描述

Axin-1 is constitutively expressed and acts as a negative regulator of Wnt expression. It has been demonstrated that mouse Wnt, Wnt3a, is secreted by multiple cell lines and can act and as a growth factor for stem cells. Secreted Wnt3a is highly hydrophobic because it is palmitoylated on an essential cysteine C77; the palmitoylated state of Wnt3a is essential for stem cell signaling. Incubation of stem cells with Wnt3a in tissue culture medium resulted in the increased number of mouse hematopoietic stem cells expressing "primitive" phenotype.

特异性

This antibody recognizes the N-terminus of Axin-1.

免疫原

GST-tagged recombinant protein corresponding to the N-terminus of mouse Axin-1.

应用

Detect Axin-1 using this Anti-Axin-1 Antibody, clone A5 validated for use in WB, IP & IC.

质量

Evaluated by Western Blot using immunoprecipitated Axin-1 in SW-480 cell lysate.
Western Blot Analysis: 1 ?g/mL of this antibody detected Axin-1 immunoprecipitated from 10 ?g of SW-480 cell lysate.

目标描述

~ 97 kDa

外形

Protein G Purified
Format: Purified

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified antibody
antibody product type	primary antibodies
克隆	A5, monoclonal
species reactivity	human
species reactivity (predicted by homology)	mouse (based on 100% sequence homology)
technique(s)	immunocytochemistry: suitable immunoprecipitation (IP): suitable western blot: suitable
同位素/亚型	IgG2aκ
NCBI登记号	NP_033863.2
UniProt登记号	O35625
运输	wet ice

安全信息

储存分类代码	12 - Non Combustible Liquids
WGK	WGK 1
闪点(F)	Not applicable
闪点(C)	Not applicable