Anti-α-Tubulin Antibody, clone DM1A

半岛bd体育手机客户端 说明

一般描述

Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein which consists of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22 nm diameter. Tyrosine ligase ads a C-terminal tyrosin to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation which is characteristic for microtubules in neuronal cells and the mitotic spindle.

特异性

Recognizes α-Tubulin, clone DM1A, MW 50-60 kDa. The epitope recognized by this antibody has been mapped to amino acids 426-450.

免疫原

Epitope: a.a. 426-450
Purified human brain α-Tubulin. The epitope recognized by this antibody has been mapped to amino acids 426-450 (AALEKDYEEVGVDSVEGEGEEEGEE).

应用

Research Category
Cell Structure
Immunofluorescence:
(Breitling, F., 1986)
Anti-α-Tubulin Antibody, clone DM1A is an antibody against α-Tubulin for use in IC, IF & WB.
Research Sub Category
Cytoskeleton

质量

Routinely evaluated by western blot on non-stimulated A431 lysate.
Western Blot Analysis:
0.1-1 ?g/mL of this lot detected α- Tubulin in RIPA lysates from A431, L6 cells and rat whole brain tissue.

目标描述

50-60 kDa

联系

Replaces: 04-1117

外形

Purified mouse monoclonal IgG1 in buffer containing 0.1M Tris-Glycine, 0.15M NaCl, 0.05% Sodium Azide and 30% glycerol.
Format: Purified
Protein G Purified

储存及稳定性

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variabillity in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

分析说明

Control
A431 cell lysate.

其他说明

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

法律信息

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

基本信息

eCl@ss	32160702
NACRES	NA.41

半岛bd体育手机客户端 性质

质量水平	100
生物来源	mouse
抗体形式	purified immunoglobulin
antibody product type	primary antibodies
克隆	DM1A, monoclonal
species reactivity	pig, human, mouse, rat, bovine, avian, guinea pig
manufacturer/tradename	Upstate?
technique(s)	immunocytochemistry: suitable immunofluorescence: suitable western blot: suitable
同位素/亚型	IgG1
NCBI登记号	NP_006000
UniProt登记号	Q71U36
运输	dry ice
Gene Information	human ... TUBA1A(7846)

安全信息

储存分类代码	10 - Combustible liquids
WGK	WGK 1